Shu Haihua, Arita Hideko, Hayashida Masakazu, Sekiyama Hiroshi, Hanaoka Kazuo
Department of Anesthesiology, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
J Ethnopharmacol. 2006 Feb 20;103(3):398-405. doi: 10.1016/j.jep.2005.08.017. Epub 2005 Sep 19.
Processed Aconiti tuber (PAT) is a herbal medicine that has been widely used as an analgesic since ancient times. We investigated effects of subanalgesic doses of PAT on morphine tolerance in mice. Mice received subcutaneous morphine (10 mg/kg) and oral PAT at subanalgesic doses (0.1 or 0.3 g/kg), once a day for 7 days. Mechanical nociceptive thresholds were measured using the tail pressure test, at 60 min after the daily s.c. morphine injections. In the placebo-treated group, repeated administration of s.c. morphine resulted in development of analgesic tolerance. In the PAT-treated groups, oral PAT attenuated morphine tolerance, dose-dependently. The main ingredient alkaloid of PAT causing its tolerance-attenuating activity was mesaconitine, but other ingredient alkaloids, such as aconitine and hypaconitine, also contributed to this activity. In addition, repeated treatment with PAT could reverse already-developed morphine tolerance. Subanalgesic doses of oral PAT thus can attenuate and reverse morphine tolerance in mice.
制川乌是一种自古以来就被广泛用作镇痛药的草药。我们研究了亚镇痛剂量的制川乌对小鼠吗啡耐受性的影响。小鼠皮下注射吗啡（10mg/kg），并口服亚镇痛剂量（0.1或0.3g/kg）的制川乌，每天一次，持续7天。在每日皮下注射吗啡60分钟后，使用尾压试验测量机械性伤害感受阈值。在安慰剂治疗组中，重复皮下注射吗啡导致镇痛耐受性的形成。在制川乌治疗组中，口服制川乌剂量依赖性地减轻了吗啡耐受性。导致其耐受性减轻活性的制川乌主要成分生物碱是中乌头碱，但其他成分生物碱，如乌头碱和次乌头碱，也对此活性有贡献。此外，重复用制川乌治疗可以逆转已经形成的吗啡耐受性。因此，亚镇痛剂量的口服制川乌可以减轻和逆转小鼠的吗啡耐受性。
