Francke Christof, Siezen Roland J, Teusink Bas
Wageningen Centre for Food Sciences, PO Box 557, 6700 AN Wageningen, the Netherlands.
Trends Microbiol. 2005 Nov;13(11):550-8. doi: 10.1016/j.tim.2005.09.001. Epub 2005 Sep 19.
The prospect of understanding the relationship between the genome and the physiology of an organism is an important incentive to reconstruct metabolic networks. The first steps in the process can be automated and it does not take much effort to obtain an initial metabolic reconstruction from a genome sequence. However, such a reconstruction is certainly not flawless and correction of the many imperfections is laborious. It requires the combined analysis of the available information on protein sequence, phylogeny, gene-context and co-occurrence but is also aided by high-throughput experimental data. Simultaneously, the reconstructed network provides the opportunity to visualize the "omics" data within a relevant biological functional context and thus aids the interpretation of those data.
理解生物体基因组与生理学之间关系的前景是重建代谢网络的一个重要动力。该过程的最初步骤可以自动化，而且从基因组序列获得初始代谢重建并不需要太多努力。然而，这样的重建肯定并非完美无缺，纠正众多缺陷很费力。这需要对蛋白质序列、系统发育、基因背景和共现的现有信息进行综合分析，但高通量实验数据也能提供帮助。同时，重建的网络提供了在相关生物学功能背景下可视化“组学”数据的机会，从而有助于对这些数据的解释。
