Smailus Duane E, Marziali Andre, Dextras Philip, Marra Marco A, Holt Robert A
Canada's Michael Smith Genome Sciences Centre, British Columbia Cancer Agency, Vancouver, British Columbia, Canada V5Z 4S6.
Genome Res. 2005 Oct;15(10):1447-50. doi: 10.1101/gr.4221805. Epub 2005 Sep 16.
We have developed high-throughput DNA sequencing methods that generate high quality data from reactions as small as 400 nL, providing an approximate order of magnitude reduction in reagent use relative to standard protocols. Sequencing of clones from plasmid, fosmid, and BAC libraries yielded read lengths (PHRED20 bases) of 765 +/- 172 (n = 10,272), 621 +/- 201 (n = 1824), and 647 +/- 189 (n = 568), respectively. Implementation of these procedures at high-throughput genome centers could have a substantial impact on the amount of data that can be generated per unit cost.
我们已经开发出高通量DNA测序方法,该方法能从低至400纳升的反应中生成高质量数据,与标准方案相比,试剂用量减少了大约一个数量级。对来自质粒、fosmid和BAC文库的克隆进行测序,得到的读长(PHRED20碱基)分别为765±172(n = 10272)、621±201(n = 1824)和647±189(n = 568)。在高通量基因组中心实施这些程序可能会对单位成本可生成的数据量产生重大影响。