Pincet Frédéric, Husson Julien
Laboratoire de Physique Statistique de l'Ecole Normale Supérieure, Paris, France.
Biophys J. 2005 Dec;89(6):4374-81. doi: 10.1529/biophysj.105.067769. Epub 2005 Sep 16.
In the past few years, many studies have attempted to measure the strength of a single molecular bond. In general, these experiments consisted in pulling on the bond and measuring the force necessary to dissociate the molecules. However, seemingly contradictory experimental results led to draw the intriguing conclusion that the strength of the bond could depend on the experiment even if the pulling conditions are similar: this paradox was first observed on the widely used streptavidin-biotin bond. Here, by doing supplementary measurements and by reanalyzing the controversial experimental results using Kramers' theory, we show that they can be conciliated. This allows us to show that the strength of a bond is very sensitive to the history of its formation, which is the key to the paradox.
在过去几年中，许多研究试图测量单个分子键的强度。一般来说，这些实验包括拉伸键并测量使分子解离所需的力。然而，看似矛盾的实验结果得出了一个有趣的结论，即即使拉伸条件相似，键的强度也可能取决于实验：这个悖论最早在广泛使用的链霉亲和素-生物素键上被观察到。在这里，通过进行补充测量并使用克莱默斯理论重新分析有争议的实验结果，我们表明它们可以得到调和。这使我们能够证明键的强度对其形成历史非常敏感，这就是悖论的关键所在。
