Sato S
Department of Biology, Faculty of Science, Ehime University, Japan.
Biotech Histochem. 1992 May;67(3):149-52. doi: 10.3109/10520299209110025.
A simple silver staining technique was applied to serial ultrathin sections of Vicia faba root tip meristems to investigate the structural organization of the nucleoli. The procedure consists of three steps: hydrophilic Lowicryl resin embedding, preparation of ribbons of serial ultrathin sections, and silver impregnation. This technique provided good contrast for a filamentous structure, previously called the "nucleolonema", in nucleoli at the light microscope level. Silver appears to react with some acidic proteins involved with ribosomal RNA transcription, since the major constituents of the nucleolonema are believed to be active rRNA transcriptional units. Tracing the nucleolonema through serial ultrathin sections strongly suggests that the nucleolonema is meandering and partly coiling within the nucleolus. The present technique permits identification of the nucleolonema in other plant species and investigation of its three-dimensional structural organization.
一种简单的银染技术应用于蚕豆根尖分生组织的连续超薄切片，以研究核仁的结构组织。该程序包括三个步骤：亲水性Lowicryl树脂包埋、连续超薄切片带的制备和银浸染。这项技术在光学显微镜水平上为核仁中一种以前称为“核仁线”的丝状结构提供了良好的对比度。银似乎与一些参与核糖体RNA转录的酸性蛋白质发生反应，因为核仁线的主要成分被认为是活跃的rRNA转录单位。通过连续超薄切片追踪核仁线强烈表明，核仁线在核仁内蜿蜒并部分盘绕。目前的技术允许在其他植物物种中识别核仁线并研究其三维结构组织。
