一种从不同植物物种中提取DNA的快速、简单且可靠的高产方法。

A fast, simple, and reliable high-yielding method for DNA extraction from different plant species.

作者信息

Tapia-Tussell Raul, Quijano-Ramayo Andres, Rojas-Herrera Rafael, Larque-Saavedra Alfonso, Perez-Brito Daisy

机构信息

Laboratorio GeMBio, Centro de Investigación Científica de Yucatán, Calle 43, #130, Col. Chuburná de Hidalgo, Mérida 97200, Yucatán, México.

出版信息

Mol Biotechnol. 2005 Oct;31(2):137-9. doi: 10.1385/MB:31:2:137.

DOI:10.1385/MB:31:2:137

PMID:16170214

Abstract

Genetic studies and pathogen detection in plants using molecular methods require the isolation of DNA from a large number of samples in a short time span. A rapid and versatile protocol for extracting high-quality DNA from different plant species is described. This method yields from 1 to 2 mg of DNA per gram of tissue. The absorbance ratios (A260/A280) obtained ranged from 1.6 to 2.0. A minimal presence of contaminating metabolites (as polymerase chain reaction [PCR] inhibitors) in samples and a considerable savings in reagents are characteristics of this protocol, as well as the low cost of the analysis per sample. The quality of the DNA was suitable for PCR amplification.

摘要

利用分子方法对植物进行遗传研究和病原体检测，需要在短时间内从大量样本中分离DNA。本文描述了一种从不同植物物种中快速提取高质量DNA的通用方法。该方法每克组织可提取1至2毫克DNA。所获得的吸光度比值（A260/A280）在1.6至2.0之间。样本中污染性代谢物（作为聚合酶链反应[PCR]抑制剂）的含量极低，试剂用量大幅节省，每个样本的分析成本也很低，这些都是该方法的特点。所提取DNA的质量适合进行PCR扩增。