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利用分子标记探索墨西哥罗克斯伯氏植物的遗传变异性以建立高效改良计划。

Exploring the Genetic Variability of Roxb. in Mexico with Molecular Markers to Establish an Efficient Improvement Program.

作者信息

Ortega-Ramírez Marynor E, Magaña-Álvarez Anuar, Pérez-Brito Daisy, Cortés-Velázquez Alberto, Nexticapan-Garcéz Ángel, Tapia-Tussell Raúl, Martín-Mex Rodolfo

机构信息

Facultad de Ciencias Agropecuarias-CV, Universidad Autónoma de Chiapas, Carretera Ocozocoautla, Villaflores 30470, Chiapas, Mexico.

GeMBio, Grupo de Estudios Moleculares Aplicados a la Biología, Centro de Investigación Científica de Yucatán A.C., Calle 43 # 130, x 32 y 34, Chuburná de Hidalgo, Mérida 97205, Yucatán, Mexico.

出版信息

Plants (Basel). 2025 Jun 19;14(12):1888. doi: 10.3390/plants14121888.

DOI:10.3390/plants14121888
PMID:40573876
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12196935/
Abstract

Melina ( Roxb.) is a tree native to Asia, whose timber is not utilized in that region for a variety of reasons. However, the tree's fast growth and extensive range of applications have increased its acceptance in other world'regions. was introduced to Mexico in 1971, and it is currently the fifth most utilized forest species in commercial forest plantations (CFPs). However, its genetic diversity has not been evaluated in Mexico. The objective of this research was to investigate the genetic variability of Melina in Mexico using molecular markers. This investigation was undertaken to acquire valuable insights for the implementation of effective improvement strategies. A total of 85 Melina samples were collected from various locations in southeastern Mexico between 2017 and 2022. Genetic fingerprints were obtained using ten simple primer amplification reactions (SPARs): five Directed Amplification of Minisatellite DNA regions (DAMD), and five Inter-Simple Sequence Repeats (ISSRs). The polymorphic information content (PIC) was 0.940 and 0.950 for the DAMD and ISSR, respectively, and the similarity coefficients ranged from 0.12 to 0.88, indicating a high degree of polymorphism in the species under investigation. This is the first attempt to ascertain the genetic variability of in Mexico.

摘要

桃花心木(Melina (Roxb.))是一种原产于亚洲的树木,由于多种原因,该地区并未利用其木材。然而,这种树生长迅速且应用广泛,在世界其他地区越来越受到认可。它于1971年被引入墨西哥,目前是商业人工林中使用量排名第五的森林树种。然而,墨西哥尚未对其遗传多样性进行评估。本研究的目的是利用分子标记研究墨西哥桃花心木的遗传变异性。开展这项调查是为了获得有价值的见解,以实施有效的改良策略。2017年至2022年期间,从墨西哥东南部的不同地点共采集了85个桃花心木样本。使用十种简单引物扩增反应(SPARs)获得遗传指纹:五种小卫星DNA区域定向扩增(DAMD)和五种简单序列重复区间(ISSR)。DAMD和ISSR的多态信息含量(PIC)分别为0.940和0.950,相似系数范围为0.12至0.88,表明所研究的物种具有高度多态性。这是在墨西哥确定桃花心木遗传变异性的首次尝试。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/938b/12196935/8e6bd38ba1b9/plants-14-01888-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/938b/12196935/e0068922ae60/plants-14-01888-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/938b/12196935/48bac59d3b65/plants-14-01888-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/938b/12196935/8e6bd38ba1b9/plants-14-01888-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/938b/12196935/e0068922ae60/plants-14-01888-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/938b/12196935/48bac59d3b65/plants-14-01888-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/938b/12196935/8e6bd38ba1b9/plants-14-01888-g003.jpg

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本文引用的文献

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Genetic relationships among wild and cultivated accessions of curry leaf plant (Murraya koenigii (L.) Spreng.), as revealed by DNA fingerprinting methods.利用 DNA 指纹图谱技术揭示野生和栽培咖喱叶植物(Murraya koenigii (L.) Spreng.)品种间的遗传关系。
Mol Biotechnol. 2013 Feb;53(2):139-49. doi: 10.1007/s12033-012-9500-4.
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Genome. 1993 Feb;36(1):181-6. doi: 10.1139/g93-024.
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A fast, simple, and reliable high-yielding method for DNA extraction from different plant species.
一种从不同植物物种中提取DNA的快速、简单且可靠的高产方法。
Mol Biotechnol. 2005 Oct;31(2):137-9. doi: 10.1385/MB:31:2:137.