Espinoza-Ulloa Sebastian A
Facultad de Medicina Pontificia Universidad Católica del Ecuador Quito Pichincha Ecuador.
Department of Biology University of Saskatchewan Saskatoon SK Canada.
Ecol Evol. 2021 May 11;11(12):7946-7950. doi: 10.1002/ece3.7624. eCollection 2021 Jun.
The young leaves are the main source of nucleic acids for population genetic studies in palm-trees; however, the access to this tissue may be limited by specific features of each species. Using root tissues as an alternative source of nucleic acids could facilitate the sampling in large populations.This study tests root tissue viability as an alternative nucleic acid source (root versus. leaf) and explores different protocols (tissue storage and DNA extraction methods) to obtain high-quality DNA samples.The results showed no significant differences in DNA concentration (603.7 vs. 599.1 ng/μl) and quality ratios (A260/280:2.1 vs. 1.9, and A260/230:2.1 vs. 2.0) for the comparisons of tissue source (leaf vs. root) and DNA extraction method (manual vs. kit). For tissue storage method, DNA concentration was significantly higher for root tissues stored in 70% and 90% alcohol solutions (692.8 and 822.6 ng/μl, respectively) versus those obtained from leaf tissue (603.7 ng/μl); however, for the quality parameters, no differences were found.Results showed the effective potential of using root tissue as an alternative source for nucleic acids, which could facilitate population sampling of palm-tree species for future studies, and this methodological alternative could be applied to other plant systems with similar sampling challenges. .
嫩叶是棕榈树群体遗传学研究中核酸的主要来源；然而，获取该组织可能会受到每个物种特定特征的限制。使用根组织作为核酸的替代来源可以方便在大群体中进行采样。本研究测试了根组织作为替代核酸来源（根与叶）的活力，并探索了不同的方案（组织储存和DNA提取方法）以获得高质量的DNA样本。结果表明，在组织来源（叶与根）和DNA提取方法（手动与试剂盒）的比较中，DNA浓度（603.7对599.1 ng/μl）和质量比（A260/280：2.1对1.9，以及A260/230：2.1对2.0）没有显著差异。对于组织储存方法，储存在70%和90%酒精溶液中的根组织的DNA浓度（分别为692.8和822.6 ng/μl）显著高于从叶组织获得的DNA浓度（603.7 ng/μl）；然而，在质量参数方面，未发现差异。结果显示了使用根组织作为核酸替代来源的有效潜力，这可以方便未来对棕榈树种进行群体采样，并且这种方法替代方案可以应用于其他面临类似采样挑战的植物系统。
