Simon Anne, Durrieu Marie-Christine
INSERM U577 Biomaterials and tissue repair, Université Victor Segalen Bordeaux 2, France.
Micron. 2006;37(1):1-13. doi: 10.1016/j.micron.2005.06.006. Epub 2005 Jul 27.
Atomic Force Microscopy (AFM) provides a range of strategies for investigating living cell adhesion to the extracellular matrix, other cells or biomaterials in their native environment. This review surveys the results obtained from major studies using AFM for mechanical force evaluation in the cell, morphological visualization of the cell and studies of the cell's response to chemical or mechanical stress. Recently, the use of AFM has been broadened to obtain experimental information about cell adhesion molecules. Quantitative measurements of binding forces between adhesion proteins and their ligands in the cell or on a surface are presented. These analyses provide data on individual molecules and their resulting collective behaviour at the cell level. They significantly contribute to the characterisation of cellular adhesion with physical principles relating to biochemistry.
原子力显微镜（AFM）提供了一系列策略，用于研究活细胞在其天然环境中与细胞外基质、其他细胞或生物材料的粘附。本综述概述了使用AFM进行细胞机械力评估、细胞形态可视化以及细胞对化学或机械应力反应研究的主要研究成果。最近，AFM的应用范围已扩大到获取有关细胞粘附分子的实验信息。文中介绍了对细胞内或表面粘附蛋白与其配体之间结合力的定量测量。这些分析提供了关于单个分子及其在细胞水平上产生的集体行为的数据。它们极大地促进了利用与生物化学相关的物理原理对细胞粘附进行表征。
