Zhu Xinyao, Zhang Nan, Wang Zuobin, Liu X
School of Engineering, University of Warwick, Coventry, CV4 7AL UK.
International Research Centre for Nano Handling and Manufacturing, Changchun University of Science and Technology, 7089 Weixing Road, Changchun, 130022 People's Republic of China.
J Microbio Robot. 2016;11(1):47-55. doi: 10.1007/s12213-016-0089-8. Epub 2016 May 7.
In this study, we presented an investigation of mechanical properties by AFM nanoindentation on human hepatocellular carcinoma cells treated with fullerenol for 24, 48 and 72 h. AFM nanoindentation was routinely applied to investigate the morphology and biomechanical properties of living carcinoma cells, and adhesion phenomena (negative force) were detected in the obtained force-displacement curves. Conventionally, Hertz contact model has been widely used for determination of cell elasticity, however this contact model cannot account for adhesion. Alternatively, JKR contact model, as expected for adhesion circumstance, has been applied to fit the obtained force-displacement curves. In this investigation, we have derived both the work of adhesion and the elastic modulus of biological cells (human hepatocellular carcinoma) under fullerenol treatment. The results show that the chosen JKR model can provide better fitting results than Hertz contact model. The results show that both Young's modulus and work of adhesion exhibit significant variation as the treatment time increases. The calculated mechanical properties of elastic modulus and work of adhesion can be used as an effective bio-index to evaluate the effects of fullerenol or other anticancer agents on cancer cells and thus to provide insight into cancer progression in the treatment.
在本研究中，我们对用富勒醇处理24、48和72小时的人肝癌细胞进行了原子力显微镜纳米压痕力学性能研究。原子力显微镜纳米压痕常用于研究活癌细胞的形态和生物力学性能，并且在获得的力-位移曲线中检测到了粘附现象（负力）。传统上，赫兹接触模型已被广泛用于确定细胞弹性，然而该接触模型无法解释粘附现象。另外，正如粘附情况所预期的那样，JKR接触模型已被用于拟合获得的力-位移曲线。在本研究中，我们得出了富勒醇处理下生物细胞（人肝癌细胞）的粘附功和弹性模量。结果表明，所选的JKR模型比赫兹接触模型能提供更好的拟合结果。结果表明，随着处理时间的增加，杨氏模量和粘附功均表现出显著变化。计算得到的弹性模量和粘附功的力学性能可作为一种有效的生物指标，用于评估富勒醇或其他抗癌药物对癌细胞的作用，从而深入了解治疗过程中的癌症进展情况。
