通过芘衍生物的双荧光监测DNA结构
Monitoring DNA structures by dual fluorescence of pyrene derivatives.
作者信息
Okamoto Akimitsu, Tainaka Kazuki, Nishiza Ken-ichiro, Saito Isao
机构信息
Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, Kyoto 615-8510, Japan.
出版信息
J Am Chem Soc. 2005 Sep 28;127(38):13128-9. doi: 10.1021/ja053609e.
We have developed a nucleotide modified by a pyrene derivative with dual fluorescence. The dual fluorescence of the fluorophore, which was incorporated into DNA, was effectively controlled at ambient temperature according to DNA structural status. Our nucleoside with dual fluorescence is effective as a conceptually new probe for monitoring DNA hybridization by the color change without multilabeling with fluorescent dyes.
我们开发了一种由芘衍生物修饰的具有双荧光的核苷酸。根据DNA的结构状态，在室温下可有效控制掺入DNA中的荧光团的双荧光。我们的双荧光核苷作为一种概念上全新的探针，可通过颜色变化监测DNA杂交，而无需用荧光染料进行多标记。
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