[PEMT2过表达对大鼠肝癌细胞中不同PKC亚型表达及转位的影响]
[Effects of overexpression of PEMT2 on expression and translocation of different PKC isoforms in rat hepatoma cells].
作者信息
Li Ya-li, Ma Ke-li, Zou Wei, Xia Quan, Cui Zhao-chun
机构信息
Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian 116027, China.
出版信息
Zhonghua Gan Zang Bing Za Zhi. 2005 Sep;13(9):678-81.
OBJECTIVE
To explore the mechanism of cell proliferation inhibition by transfecting phosphatidylethanolamine N-methyltransferase 2 gene (PEMT2).
METHODS
The expression and translocation of different isoforms of protein kinase C (PKC) in cells were observed with immunocytochemistry and Western blot techniques. The content of diacylglycerol (DAG) was analyzed with high performance thin layer chromatography (HPTLC) technique.
RESULTS
Transfection of PEMT2 can inhibit the expression of cPKC alpha, but obviously promotes the expression and translocation from cytosol to plasma membrane of cPKC beta2. At the same time, the content of DAG was decreased in the transfected cells. Expression and translocation of other PKC isoforms were not changed by PEMT2 transfection.
CONCLUSION
Effects of overexpression of PEMT2 on the expression and translocation of different PKC isoforms might be related to the mechanism of cell proliferation inhibition and apoptosis induced by transfecting PEMT2.
目的
通过转染磷脂酰乙醇胺N -甲基转移酶2基因（PEMT2）探讨细胞增殖抑制机制。
方法
采用免疫细胞化学和蛋白质印迹技术观察细胞中蛋白激酶C（PKC）不同亚型的表达及转位情况。利用高效薄层层析（HPTLC）技术分析二酰基甘油（DAG）含量。
结果
转染PEMT2可抑制cPKCα的表达，但明显促进cPKCβ2从胞质溶胶向质膜的表达及转位。同时，转染细胞中DAG含量降低。PEMT2转染未改变其他PKC亚型的表达及转位。
结论
PEMT2过表达对不同PKC亚型表达及转位的影响可能与转染PEMT2诱导的细胞增殖抑制和凋亡机制有关。
Zotero 插件