[ pemt - 2 - cDNA转染抑制大鼠CBRH - 7919肝癌细胞中细胞周期相关蛋白的表达]

[Transfection of pemt-2-cDNA inhibits the expression of cell cycle related proteins in rat CBRH-7919 hepatoma cells].

作者信息

Liu Cui-ping, Zou Wei, Wang Liang, Cui Zhao-chun

机构信息

Department of Biology, Liaoning Normal University, Dalian 116029, China.

出版信息

Zhonghua Gan Zang Bing Za Zhi. 2006 May;14(5):350-2.

PMID:16732909

Abstract

OBJECTIVES

To unravel the molecular mechanism of proliferation inhibition induced by transfection of pemt2-cDNA into rat CBRH-7919 hepatoma cells.

METHODS

We started with the highly expressed PEMT2 clone. Cell culture and Western blotting techniques were used to examine the expression of cyclinD1/CDK4, cyclinE/CDK2, phospho-Rb, caspase-3, c-jun and caveolins.

RESULTS

Our results showed that CDK4, CDK2, phospho-Rb and c-jun were down regulated in the pemt2 highly expressed cell clone. The high expression clone of pemt2-transfected cells also showed over expression of caspase-3.

CONCLUSION

The reductions of proliferation and apoptosis of pemt2 transfected cells could be related to the G1 phase arrest induced by down-regulation of the cell cycle-associated proteins.

摘要

目的

揭示将pemt2 - cDNA转染至大鼠CBRH - 7919肝癌细胞中所诱导的增殖抑制分子机制。

方法

我们从高表达的PEMT2克隆开始。采用细胞培养和蛋白质免疫印迹技术检测细胞周期蛋白D1/细胞周期蛋白依赖性激酶4（cyclinD1/CDK4）、细胞周期蛋白E/细胞周期蛋白依赖性激酶2（cyclinE/CDK2）、磷酸化视网膜母细胞瘤蛋白（phospho - Rb）、半胱天冬酶 - 3（caspase - 3）、c - jun和小窝蛋白（caveolins）的表达。