Denkin Steven, Byrne Sean, Jie Charles, Zhang Ying
Department of Molecular Microbiology and Immunology Bloomberg School of Public Health, Johns Hopkins University, 615 N. Wolfe Street, Baltimore, MD 21205, USA.
Arch Microbiol. 2005 Nov;184(3):152-7. doi: 10.1007/s00203-005-0037-9. Epub 2005 Nov 10.
Salicylate stimulates the oxygen consumption and also induces multiple drug resistance in Mycobacterium tuberculosis. To gain insight into the mechanisms involved in the above observations, a microarray analysis of M. tuberculosis genes in response to salicylate was performed. Salicylate, besides highly inducing the 27 kD gene (Rv0560c) previously identified as highly salicylate-inducible, also caused increased transcription of a range of genes including an open reading frame (Rv0559c) that is located immediately downstream of the 27 kD gene, and some membrane/transmembrane proteins that may serve as potential efflux pumps or porins. Salicylate also caused a general shutdown of transcription and translation and energy production by down-regulating a range of genes involved in RNA and protein synthesis and ATP synthesis. The role of the salicylate-regulated genes in salicylate induced drug resistance and its unique effect on stimulating oxygen consumption in tubercle bacillus is discussed.
水杨酸盐可刺激结核分枝杆菌的耗氧量，并诱导其产生多重耐药性。为深入了解上述现象背后的机制，我们对结核分枝杆菌响应水杨酸盐的基因进行了微阵列分析。水杨酸盐除了高度诱导先前已确定为高水杨酸盐诱导型的27 kD基因（Rv0560c）外，还导致一系列基因转录增加，包括位于27 kD基因紧邻下游的一个开放阅读框（Rv0559c），以及一些可能作为潜在外排泵或孔蛋白的膜/跨膜蛋白。水杨酸盐还通过下调一系列参与RNA和蛋白质合成以及ATP合成的基因，导致转录、翻译和能量产生的全面停止。本文讨论了水杨酸盐调控基因在水杨酸盐诱导的耐药性中的作用及其对结核杆菌耗氧刺激的独特影响。
