Fontán Patricia A, Aris Virginie, Alvarez María E, Ghanny Saleena, Cheng Jeff, Soteropoulos Patricia, Trevani Analia, Pine Richard, Smith Issar
Public Health Research Institute, International Center for Public Health, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark, NJ 07103-3535, USA.
J Infect Dis. 2008 Sep 15;198(6):877-85. doi: 10.1086/591098.
Mycobacterium tuberculosis survives in macrophages and usually subverts the bactericidal mechanisms of these phagocytes. The understanding of this host-pathogen interaction is relevant for the development of new treatments for tuberculosis. The adaptation of M. tuberculosis to intracellular life depends on its ability to regulate the expression of its genes. Sigma factors are important bacterial transcription activators that bind to the RNA polymerase and give it promoter specificity. Sigma factor E (SigE) controls the expression of genes that are essential for virulence. We have identified the SigE regulon during infection of macrophages, and we analyzed the impact of this regulon on the transcriptional response of phagocytes. Our results indicate that SigE regulates the expression of genes involved in the maintenance of M. tuberculosis cell envelope integrity and function during macrophage infection. Analysis of the phagocytes' transcriptional response indicates that the SigE regulon is involved in the modulation of the inflammatory response.
结核分枝杆菌在巨噬细胞中存活，并且通常会破坏这些吞噬细胞的杀菌机制。对这种宿主-病原体相互作用的理解与开发结核病新疗法相关。结核分枝杆菌对细胞内生活的适应取决于其调节基因表达的能力。西格玛因子是重要的细菌转录激活因子，可与RNA聚合酶结合并赋予其启动子特异性。西格玛因子E（SigE）控制对毒力至关重要的基因的表达。我们已经确定了巨噬细胞感染期间的SigE调控子，并分析了该调控子对吞噬细胞转录反应的影响。我们的结果表明，SigE在巨噬细胞感染期间调节参与维持结核分枝杆菌细胞壁完整性和功能的基因的表达。对吞噬细胞转录反应的分析表明，SigE调控子参与炎症反应的调节。
