Thouvenot D R, Morfin R F
J Chromatogr. 1979 Feb 11;170(1):165-73. doi: 10.1016/s0021-9673(00)84248-x.
A procedure is described for the quantitation of zearalenone from corn by gas-liquid chromatography. An internal standard, zearalanone, is first mixed with the finely ground corn, followed by extraction with ethyl acetate, purification by thin-layer chromatography and formation of trimethyl silyl ether (TMS) and methyl oxime (MOX) derivatives. Positive identification of zearalenone is based upon the retention times of the TMS and MOX-TMS derivatives being identical with those of the standards, comparison of chromatograms from both derivatives and characteristic twin peaks of zearalenone MOX-TMS. The detection limit of 100 parts per 10(9) zearalenone in corn could only be improved by modification of the described procedure.
描述了一种通过气液色谱法对玉米中玉米赤霉烯酮进行定量分析的方法。首先将内标物玉米赤霉酮与精细研磨的玉米混合，然后用乙酸乙酯萃取，通过薄层色谱法纯化，并形成三甲基硅醚（TMS）和甲基肟（MOX）衍生物。玉米赤霉烯酮的阳性鉴定基于TMS和MOX - TMS衍生物的保留时间与标准品相同、两种衍生物色谱图的比较以及玉米赤霉烯酮MOX - TMS的特征双峰。只有通过修改所述方法才能提高玉米中每10⁹份含100份玉米赤霉烯酮的检测限。
