Phenomenon of hepatic overload of copper in Mugil cephalus: role of metallothionein and patterns of copper cellular distribution.

In this work we describe a phenomenon of accumulation of copper (Cu) in livers of a teleost fish commonly known as mullet, Mugil cephalus. High levels of Cu, up to 1936 microg/g wet weight were found. The high Cu levels seem not to be associated with environmental Cu contamination, since the fish were collected from widely separated regions with low Cu concentrations. Other fish species sharing the same environment did not show high levels of Cu. The accumulation of Cu in mullet was seen in liver and most of the hepatic Cu was located in the non-cytosolic fraction. The intrahepatic distribution of Cu in mullet seems to depend on the total Cu content in the liver; as the total liver burden of Cu rose, Cu was increasingly recovered from the non-cytosolic fraction. Metallothionein in hepatic cytosols from mullet contained the most Cu. However, the Cu concentration not bound to metallothionein rose when total cytosolic Cu increased; which show that metallothionein, particularly at higher Cu levels, is not the major hepatic Cu-binding protein in cytosols of mullet. This report shows mullet as a very useful model to study the accumulation of Cu in the liver, which may lead to a better understanding of cellular mechanisms which control Cu homeostasis.