Jones A K, Marshall J, Blake A D, Buckingham S D, Darlison M G, Sattelle D B
MRC Functional Genetics Unit, Department of Human Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1, 3QX, UK.
Invert Neurosci. 2005 Nov;5(3-4):147-55. doi: 10.1007/s10158-005-0007-6. Epub 2005 Oct 24.
The cloning, sequencing and functional expression of Sgbeta1, a novel locust (Schistocerca gregaria) non-alpha nicotinic acetylcholine receptor (nAChR) subunit is described. This subunit shows 80% identity with the Drosophila melanogaster Dbeta1 and 92% identity with the Locusta migratoria beta1, non-alpha subunits but only 38% identity to Sgalpha1 (also referred to as alphaL1), a previously cloned S. gregaria nAChR alpha-subunit. When expressed in Xenopus laevis oocytes, Sgbeta1 does not respond to nicotine. Responses to nicotine are observed, however, in oocytes co-expressing Sgalpha1 and Sgbeta1, but the pharmacology is indistinguishable from that of currents produced by expressing Sgalpha1 alone. We conclude that either Sgbeta1 does not co-assemble with Sgalpha1, or that it is unable to contribute to the functional properties of the receptor, in the Xenopus oocyte expression system.
本文描述了一种新型蝗虫（沙漠蝗）非α型烟碱型乙酰胆碱受体（nAChR）亚基Sgbeta1的克隆、测序及功能表达。该亚基与黑腹果蝇Dbeta1有80%的同一性，与飞蝗beta1非α亚基有92%的同一性，但与先前克隆的沙漠蝗nAChRα亚基Sgalpha1（也称为alphaL1）只有38%的同一性。当在非洲爪蟾卵母细胞中表达时，Sgbeta1对尼古丁无反应。然而，在共表达Sgalpha1和Sgbeta1的卵母细胞中观察到了对尼古丁的反应，但其药理学特性与单独表达Sgalpha1产生的电流无法区分。我们得出结论，在非洲爪蟾卵母细胞表达系统中，要么Sgbeta1不能与Sgalpha1共同组装，要么它无法对受体的功能特性产生影响。
