[尾加压素II及G蛋白偶联受体14 mRNA在人嗜铬细胞瘤组织中的表达]
[Expression of urotensin II and G-protein coupled receptor 14 mRNA in human pheochromocytoma tissues].
作者信息
Liu Guo-Qiang, Zeng Zheng-Pei, Li Han-Zhong, Fan Xin-Rong, Liu Dong-Mei, Tong An-Li, Zheng Xin, Liu Chang
机构信息
Department of Endocrinology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
出版信息
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2005 Aug;27(4):457-60.
OBJECTIVE
To investigate the expression of urotensin II (U II) and G-protein coupled receptor 14 (GPR14) mRNA in human pheochromocytoma tissues.
METHODS
Total RNA from normal adrenal and pheochromocytoma tissues was extracted. The reverse transcription-polymerase chain reaction method was used to evaluate the levels of U II and GPR14 mRNA expression in human pheochromocytoma tissues.
RESULTS
There was no significant difference of U II and GPR14 mRNA expression between normal adrenal cortex and medulla. The expression of U II and GPR14 mRNA in pheochromocytoma was significantly lower than that in normal adrenal cortex and medulla (P < 0.05). The expression of GPR14 mRNA in adrenal pheochromocytomas was significantly lower than that of extra-adrenal pheochromocytomas (P < 0.05).
CONCLUSION
U II and GPR14 may play a role in the pathogenesis and hypertension regulating of pheochromocytoma.
目的
研究尾加压素II（U II）和G蛋白偶联受体14（GPR14）mRNA在人嗜铬细胞瘤组织中的表达。
方法
提取正常肾上腺组织和嗜铬细胞瘤组织的总RNA。采用逆转录-聚合酶链反应法评估人嗜铬细胞瘤组织中U II和GPR14 mRNA的表达水平。
结果
正常肾上腺皮质和髓质中U II和GPR14 mRNA的表达无显著差异。嗜铬细胞瘤中U II和GPR14 mRNA的表达显著低于正常肾上腺皮质和髓质（P < 0.05）。肾上腺嗜铬细胞瘤中GPR14 mRNA的表达显著低于肾上腺外嗜铬细胞瘤（P < 0.05）。
结论
U II和GPR14可能在嗜铬细胞瘤的发病机制及高血压调节中发挥作用。
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