[尾加压素II mRNA在正常肾上腺髓质及嗜铬细胞瘤组织中的表达]

[Expression of urotensin II mRNA in normal adrenal medulla and pheochromocytoma tissues].

作者信息

Liu Guo-qiang, Zeng Zheng-pei, Li Han-zhong, Fan Xin-rong, Liu Dong-mei, Tong An-li, Zheng Xin

机构信息

Department of Endocrinology, Peking Union College Hospital, Peking Union College and Chinese Academy of Medical Sciences, Beijing 100730, China.

出版信息

Zhonghua Yi Xue Za Zhi. 2003 Jul 25;83(14):1225-8.

PMID:12930635

Abstract

OBJECTIVE

To investigate the expression of human urotensin II (UII) mRNA in normal adrenal medulla and pheochromocytoma tissues.

METHODS

The total RNA of normal adrenal medulla from 9 males who died of accidents and the total RNA of pheochromocytoma tissues resected from 19 patients were extracted. The expression of UII mRNA was studied by reverse transcription-polymerase chain reaction (RT-PCR). UII and its internal control GAPDH were co-amplified in the same tube. Semi-quantitative analysis of the mRNA expression level of UII was carried out based on the ratios of the grey scale of the amplified target band to that of the control band (UII/GAPDH).

RESULTS

The amplified product of UII is a 385 bp fragment and 99.7% of the bases were the same as the sequence registered in the GenBank. The UII/GAPDH in the normal adrenal medullae and pheochromocytomas were 0.69 +/- 0.29 (n = 9) and 0.22 +/- 0.18 (n = 19) respectively (P < 0.05).

CONCLUSION

Both the normal adrenal medulla and pheochromocytoma express UII mRNA. The expression of UII mRNA is lower in pheochromocytoma than in normal adrenal medulla.

摘要

目的

研究人尾加压素II（UII）mRNA在正常肾上腺髓质和嗜铬细胞瘤组织中的表达。

方法

提取9例因意外死亡男性的正常肾上腺髓质总RNA以及19例患者手术切除的嗜铬细胞瘤组织总RNA。采用逆转录-聚合酶链反应（RT-PCR）研究UII mRNA的表达。UII及其内参甘油醛-3-磷酸脱氢酶（GAPDH）在同一管中共同扩增。根据扩增目的条带与对照条带灰度值之比（UII/GAPDH）对UII mRNA表达水平进行半定量分析。