[不同培养基对大鼠海马切片活力及tau蛋白表达的影响]
[Effect of different culture media on viability and tau protein expression in rat hippocampal slices].
作者信息
Zhang Ying-Chun, Liao Xiao-Mei, Wang Jian-Zhi
机构信息
Department of Pathophysiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
出版信息
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2005 Aug;27(4):513-7.
OBJECTIVE
To explore the effect of different culture media on viability and expression of tau protein in organotypic hippocampal slice.
METHODS
Brain slices (400 microm) from 1, 2, 4, and 8 week-old Wistar rats were prepared and cultured in minimum essential medium (MEM) or Dulbecco's modified eagle medium: nutrient mixture (DMEM/F12) medium respectively for 21 days. Viability of the slices was measured by lactate dehydrogenase (LDH) assay and expression of tau protein was detected by Western blot.
RESULTS
The viability of the slices was not influenced significantly by the two different culture media, while the expression level of tau protein was significantly higher in DMEM/F12 than in MEM (P < 0.05), especially in the slices from 2 and 4 week-old rats.
CONCLUSION
The slices from 2 or 4 week-old rat hippocampi and DMEM/F12 medium may be the preferred choice for tau associated researches. An ideal Alzheimer's disease model may be established based on the results of these researches.
目的
探讨不同培养基对海马脑片神经元活力及tau蛋白表达的影响。
方法
取1、2、4、8周龄Wistar大鼠的脑片（400μm），分别置于最低必需培养基（MEM）或杜氏改良伊格尔培养基：营养混合物（DMEM/F12）中培养21天。采用乳酸脱氢酶（LDH）法检测脑片活力，蛋白质免疫印迹法检测tau蛋白表达。
结果
两种培养基对脑片活力无显著影响，但DMEM/F12培养基中tau蛋白的表达水平显著高于MEM（P<0.05），尤其是2周龄和4周龄大鼠的脑片。
结论
2或4周龄大鼠海马脑片与DMEM/F12培养基可能是tau相关研究的首选。基于这些研究结果，有望建立理想的阿尔茨海默病模型。
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