Cechetti Fernanda, Rhod Amanda, Simão Fabrício, Santin Katiane, Salbego Christianne, Netto Carlos Alexandre, Siqueira Ionara Rodrigues
Programa de Pós Graduação em Ciências Biológicas-Neurociências, Universidade Federal do Rio Grande do Sul, Rua Sarmento Leite, 500, 90050-170, Porto Alegre, RS, Brazil.
Brain Res. 2007 Jul 9;1157:121-5. doi: 10.1016/j.brainres.2007.04.045. Epub 2007 Apr 24.
We have recently demonstrated that high intensity training exercise exacerbates brain damage, while a moderate intensity (2 weeks of 20 min/day of treadmill training) reduces the injury caused by in vitro ischemia, oxygen and glucose deprivation (OGD), to hippocampal slices from Wistar rats. In the present paper, the effect of different running programs on severity of ischemic OGD lesion was examined, by the evaluation of three protocols designed to simulate exercise conditions common to humans: one or three 20-min sessions per week, during 12 weeks (moderate intensity), and two 20-min daily sessions for 3 weeks. OGD caused an increase of lactate dehydrogenase (LDH) release into the incubation media, a marker of tissue necrosis, and a decline of cell viability, as assessed by the decrease of mitochondrial dehydrogenase activity (MTT method). Moderate exercise, three times a week during 12-week treadmill training, decreased LDH release after OGD, while one weekly session and 3 weeks of two daily sessions did not affect OGD-induced LDH released. No exercise protocol evaluated altered MTT reduction. Our data support the hypothesis that moderate intensity exercise reduces hippocampal susceptibility to in vitro ischemia.
我们最近证实，高强度训练运动会加剧脑损伤，而中等强度运动（每天在跑步机上训练20分钟，持续2周）可减轻体外缺血、缺氧及无糖（OGD）对Wistar大鼠海马切片造成的损伤。在本文中，通过评估三种旨在模拟人类常见运动条件的方案，研究了不同跑步方案对缺血性OGD损伤严重程度的影响：每周进行一次或三次20分钟的训练，持续12周（中等强度），以及每天进行两次20分钟的训练，持续3周。OGD导致孵育培养基中乳酸脱氢酶（LDH）释放增加，这是组织坏死的一个指标，同时通过线粒体脱氢酶活性的降低（MTT法）评估发现细胞活力下降。在为期12周的跑步机训练中，每周进行三次中等强度运动，可降低OGD后的LDH释放，而每周进行一次训练以及每天进行两次训练、持续3周的方案则不会影响OGD诱导的LDH释放。所评估的运动方案均未改变MTT还原率。我们的数据支持这样的假设，即中等强度运动可降低海马体对体外缺血的易感性。
