Merino Francisco Jesús, Nebreda Teresa, Serrano Jose Luis, Fernández-Soto Pedro, Encinas Antonio, Pérez-Sánchez Ricardo
Servicio de Microbiología, Hospital Santa Bárbara, Complejo Hospitalario de Soria, Soria, Spain.
Epidemiol Infect. 2005 Oct;133(5):943-9. doi: 10.1017/S0950268805004061.
To determine the tick species that bite humans in the province of Soria (Spain) and ascertain the tick-borne pathogens that threaten people's health in that province, 185 tick specimens were collected from 179 patients who sought medical advice at health-care centres. The ticks were identified, and their DNA examined by PCR for pathogens. Most ticks were collected in autumn and spring (59 and 57 respectively). Nine species of ticks were identified, the most frequent being Dermacentor marginatus (55.7%), Ixodes ricinus (12.4%) and Rhipicephalus bursa (11.9%). Ninety-seven females, 66 males, 21 nymphs and one larva were identified. Twenty-six ticks carried DNA from Rickettsia spp. (11 Rickettsia slovaca, 6 Rickettsia spp. RpA4/DnS14, 1 Rickettsia massiliae/Bar29, and 8 unidentified); two ticks carried DNA from Borrelia burgdorferi sensu lato and seven ticks harboured DNA from Anaplasma phagocytophilum.
为确定西班牙索里亚省叮咬人类的蜱虫种类，并查明该省威胁人类健康的蜱传病原体，从179名到医疗保健中心寻求医疗建议的患者身上采集了185只蜱虫标本。对蜱虫进行了鉴定，并通过聚合酶链反应（PCR）检测其DNA中的病原体。大多数蜱虫是在秋季和春季采集的（分别为59只和57只）。鉴定出9种蜱虫，最常见的是边缘革蜱（55.7%）、蓖麻硬蜱（12.4%）和柏氏扇头蜱（11.9%）。鉴定出97只雌蜱、66只雄蜱、21只若蜱和1只幼虫。26只蜱虫携带立克次体属的DNA（11只斯洛伐克立克次体、6只立克次体属RpA4/DnS14、1只马赛立克次体/Bar29和8只未鉴定的）；2只蜱虫携带伯氏疏螺旋体狭义亚种的DNA，7只蜱虫携带嗜吞噬细胞无形体的DNA。
