Thorp B H, Anderson I, Jakowlew S B
AFRC Institute of Animal Physiology and Genetics, Edinburgh Research Station, Roslin, Midlothian, Scotland.
Development. 1992 Apr;114(4):907-11. doi: 10.1242/dev.114.4.907.
The localization of TGF-beta 1, -beta 2 and -beta 3 was studied in the growth plate, epiphysis and metaphysis of the tibiotarsus of three-week-old chicks. The different TGF-beta isoforms were localized to hypertrophic chondrocytes, chondroclasts, osteoblasts and osteoclasts using immunohistochemical staining analysis with specific TGF-beta antibodies. TGF-betas in osteoclasts and chondroclasts were restricted to those cells located on the respective matrices. TGF-beta 3 localization was mainly cytoplasmic in the transitional (early hypertrophic) chondrocytes, but nuclear staining was also detected in some proliferating chondrocytes. The cell-specific localization of these TGF-beta isoforms supports the hypothesis that TGF-beta has a role in the coupling of new bone formation to bone and cartilage matrix resorption during osteochondral development and suggests that TGF-beta may be a marker of chondrocyte differentiation. TGF-beta localization preceded a marked increase in type II collagen mRNA expression in transitional chondrocytes, suggesting a role for TGF-beta in the induction of synthesis of extracellular matrix.
研究了三周龄雏鸡胫跗骨生长板、骨骺和干骺端中转化生长因子β1、β2和β3的定位。使用特异性转化生长因子β抗体通过免疫组织化学染色分析，将不同的转化生长因子β亚型定位到肥大软骨细胞、破骨细胞、成骨细胞和破骨细胞。破骨细胞和破骨细胞中的转化生长因子β局限于位于各自基质上的那些细胞。转化生长因子β3在过渡性（早期肥大）软骨细胞中的定位主要在细胞质中，但在一些增殖软骨细胞中也检测到核染色。这些转化生长因子β亚型的细胞特异性定位支持了这样的假设，即转化生长因子β在骨软骨发育过程中对新骨形成与骨和软骨基质吸收的耦合中起作用，并表明转化生长因子β可能是软骨细胞分化的标志物。转化生长因子β的定位先于过渡性软骨细胞中II型胶原mRNA表达的显著增加，表明转化生长因子β在诱导细胞外基质合成中起作用。
