Thorp B H, Jakowlew S B
AFRC Roslin Institute (Edinburgh), Midlothian, Scotland.
Bone. 1994 Jan-Feb;15(1):59-64. doi: 10.1016/8756-3282(94)90892-3.
Growth plates from chicks displaying hypocalcaemic rickets, hypophosphataemic rickets and dyschondroplasia were studied. Immunohistochemical staining using specific TGF-beta 3 antibodies showed disruption of TGF-beta 3 localisation in all three disorders. In hypocalcaemic rickets, transitional and hypertrophic chondrocytes showed expression of TGF-beta 3, while accumulating, proliferative chondrocytes showed little expression. In the accumulating hypertrophic chondrocytes of hypophosphataemic rickets there was a reduction in the number of chondrocytes expressing TGF-beta 3, but transitional cells stained intensely. In the accumulating transitional chondrocytes of dyschondroplastic physes there was a reduction in the number of chondrocytes containing TGF-beta 3 and, in addition, the concentration of TGF-beta 3 appeared reduced. In all three disorders, there was localisation of some TGF-beta 3 in chondrocytes that appeared to be differentiating within the areas of repair. In addition, little TGF-beta 3 was detected in osteoclasts resorbing accumulated matrix from hypocalcaemic, hypophosphataemic and dyschondroplastic growth plates. These in vivo studies show a reduction in TGF-beta 3 localisation within growth plate chondrocytes and osteoclasts in three disorders in which chondrocytes cease to differentiate. This suggests that TGF-beta 3 may be important during chondrocyte differentiation in the growth plate.
对表现出低钙血症性佝偻病、低磷血症性佝偻病和软骨发育异常的雏鸡生长板进行了研究。使用特异性转化生长因子β3（TGF-β3）抗体的免疫组织化学染色显示，在所有这三种病症中TGF-β3的定位均受到破坏。在低钙血症性佝偻病中，过渡性和肥大性软骨细胞显示出TGF-β3的表达，而正在积聚的增殖性软骨细胞表达很少。在低磷血症性佝偻病正在积聚的肥大性软骨细胞中，表达TGF-β3的软骨细胞数量减少，但过渡性细胞染色强烈。在软骨发育异常的生长板正在积聚的过渡性软骨细胞中，含有TGF-β3的软骨细胞数量减少，此外，TGF-β3的浓度似乎也降低了。在所有这三种病症中，在修复区域内似乎正在分化的软骨细胞中存在一些TGF-β3的定位。此外，在从低钙血症性、低磷血症性和软骨发育异常的生长板中吸收积聚基质的破骨细胞中几乎未检测到TGF-β3。这些体内研究表明，在软骨细胞停止分化的三种病症中，生长板软骨细胞和破骨细胞内的TGF-β3定位减少。这表明TGF-β3在生长板软骨细胞分化过程中可能很重要。
