Otten Gillis R, Schaefer Mary, Doe Barbara, Liu Hong, Megede Jan Zur, Donnelly John, Rabussay Dietmar, Barnett Susan, Ulmer Jeffrey B
Vaccines Research, Chiron Corporation 4560 Horton St., Mail Stop 4.3, Emeryville, CA 94608, USA.
Vaccine. 2006 May 22;24(21):4503-9. doi: 10.1016/j.vaccine.2005.08.017. Epub 2005 Aug 19.
A plasmid DNA vaccine containing a fusion gene consisting of an HIV-1 subtype C gag and a modified subtype C pol was compared to a mixture of gag plus pol or gag plus HIV env plasmids. Plasmid DNA was delivered by intramuscular injection followed by electroporation in vivo. Two vaccinations were sufficient to induce high levels of Gag- and Pol-specific CD4 and CD8 T cells in peripheral blood. The gag-pol fusion plasmid was as immunogenic as the plasmid mixtures. Thus, DNA vaccination by intramuscular electroporation was an effective means for inducing high levels of Gag- and Pol-specific T cells, and a single gag-pol fusion DNA vaccine was sufficient for eliciting immune responses against both antigens.
将含有由HIV-1 C亚型gag和修饰的C亚型pol组成的融合基因的质粒DNA疫苗与gag加pol或gag加HIV env质粒的混合物进行比较。通过肌肉注射递送质粒DNA，随后在体内进行电穿孔。两次接种足以在外周血中诱导高水平的Gag和Pol特异性CD4和CD8 T细胞。gag-pol融合质粒与质粒混合物具有相同的免疫原性。因此，通过肌肉电穿孔进行DNA疫苗接种是诱导高水平Gag和Pol特异性T细胞的有效手段，单一的gag-pol融合DNA疫苗足以引发针对两种抗原的免疫反应。
