Wang Zhaohong, Wen Jiao, Xing Junbo, He Ye
Forensic Science Institute of Public Security Ministry, Toxicology Analysis, Muxidi Nanli 17, Xicheng District, Beijing 100038, PR China.
J Pharm Biomed Anal. 2006 Mar 3;40(4):1031-4. doi: 10.1016/j.jpba.2005.08.012. Epub 2005 Sep 21.
A high performance liquid chromatography (HPLC) method has been developed for the determination of five principal alkaloids (benzoylmesaconine, mesaconitine, aconitine, hypaconitine and deoxyaconitine) found in four species of genus Aconitum. The five alkaloids were analyzed simultaneously with an XTerraRP18 column by gradient elution using 0.03 M ammonium hydrogen carbonate-acetonitrile as mobile phase. The recovery of the method was 94.6-101.9%, and all the alkaloids showed good linearity (gamma = 0.9999) in a relatively wide concentration range. The results indicated that contents of alkaloids in Aconitum varied significantly from species to species; hence quality control of Aconitum drugs is very necessary.
已开发出一种高效液相色谱（HPLC）法，用于测定乌头属四种植物中发现的五种主要生物碱（苯甲酰新乌头碱、新乌头碱、乌头碱、次乌头碱和去氧乌头碱）。使用0.03M碳酸氢铵-乙腈作为流动相，通过梯度洗脱，在XTerraRP18柱上同时分析这五种生物碱。该方法的回收率为94.6-101.9%，所有生物碱在相对较宽的浓度范围内均显示出良好的线性（γ=0.9999）。结果表明，不同种乌头属植物中生物碱的含量差异显著；因此，乌头属药物的质量控制非常必要。
