Huang Jianming, Guo Jixian, Sun Mingming, Lu Bin, Duan Gengli, Chen Wansheng
School of Pharmacy, Fudan University, Shanghai 200032.
Zhong Yao Cai. 2002 Dec;25(12):878-80.
The methods for the determination of Aconitum alkaloids in Radix Aconiti Kusnezoffii were established. Volumetric analysis with the indicator of methyl red-bromcresol green and colorimetric analysis with acid dye of bromecresol green under the wavelength of 416 nm were used for the assay of total alkaloids. Colorimetric analysis of hydroxamic acid-Fe was for the assay of total ester-diterpene type Aconitum alkaloids. Three kinds of diester-diterpene type Aconitum alkaloids (aconitine, hypaconitine and mesaconitine) were determined by HPLC method. The HPLC system consisted of C18 as the stationary phase and acetonitrile: buffer (acetic acid-triethylamine, pH 6.25) = 71:29 as the mobile phase. The wavelength was 235 nm.
建立了测定草乌中乌头生物碱的方法。采用以甲基红-溴甲酚绿为指示剂的容量分析法和在416nm波长下用溴甲酚绿酸性染料进行比色分析来测定总生物碱。采用异羟肟酸-铁比色分析法测定总酯型二萜类乌头生物碱。采用高效液相色谱法测定三种二酯型二萜类乌头生物碱（乌头碱、次乌头碱和新乌头碱）。高效液相色谱系统以C18为固定相，乙腈：缓冲液（乙酸-三乙胺，pH 6.25）=71:29为流动相。检测波长为235nm。
