Van Driessche Benoit, Coddens Séverine, Van Mullem Vincent, Vandenhaute Jean
Unité de Recherche en Biologie Moléculaire, University of Namur, Belgium.
FEBS Lett. 2005 Oct 10;579(24):5318-24. doi: 10.1016/j.febslet.2005.08.057.
Ctk1 is a kinase involved in transcriptional control. We show in the two-hybrid system that Ctk1 interacts with Snf1, a kinase regulating glucose-dependent genes. Co-purification experiments confirmed the two-hybrid interaction but only when cells were grown at low glucose concentrations. Deletion of Ctk1 or its associated partners, Ctk2 and Ctk3, conferred synthetic lethality with null mutants of Snf1 or Snf1-associated proteins. Northern blot analysis suggested that Ctk1 and Snf1 act together in vivo to regulate GSY2. These findings support the view that Ctk1 interacts with Snf1 in a functional module involved in the cellular response to glucose limitation.
