Hutchings Geoffrey H, Ferris Nigel P
Institute for Animal Health, Pirbright Laboratory, Ash Road, Pirbright, Woking, Surrey GU24 0NF, UK.
J Virol Methods. 2006 Feb;131(2):213-7. doi: 10.1016/j.jviromet.2005.08.009. Epub 2005 Sep 22.
Two indirect, sandwich ELISAs are described for use in African swine fever (ASF) diagnosis. One assay uses polyclonal serum raised in rabbits and guinea pigs against the cytoplasmic soluble ASF virus protein and the second, a combination of monoclonal antibody raised against the VP73 protein and rabbit polyclonal serum. Both assays have been shown to detect antigen of representative field strains of phylogenetically distinct groupings of ASF virus but the ELISA, which utilises polyclonal antisera was slightly more sensitive than that using the monoclonal antibody.
本文描述了两种用于非洲猪瘟（ASF）诊断的间接夹心酶联免疫吸附测定（ELISA）方法。一种检测方法使用在兔子和豚鼠体内产生的针对细胞质可溶性ASF病毒蛋白的多克隆血清，另一种方法则使用针对VP73蛋白产生的单克隆抗体与兔多克隆血清的组合。两种检测方法均已证明能检测到ASF病毒系统发育不同分组的代表性野毒株的抗原，但使用多克隆抗血清的ELISA比使用单克隆抗体的方法灵敏度略高。
