Vidal M I, Stiene M, Henkel J, Bilitewski U, Costa J V, Oliva A G
Instituto de Biologia Experimental e Tecnologica (IBET)/Instituto de Tecnologia Quimica e Biológica (ITQB), Universidade Nova de Lisboa, Oeiras, Portugal.
J Virol Methods. 1997 Jul;66(2):211-8. doi: 10.1016/s0166-0934(97)00059-1.
An improved solid-phase enzyme linked immunosorbent assay (ELISA) using monoclonal antibodies was developed to detect an African swine fever virus protein (VP73) in pig samples. The use of monoclonal antibodies against VP73 allowed a sensitive and specific sandwich ELISA. This assay detected a limiting antigen concentration of 0.05 microgram/ml of VP73, lower than the detection limit of 0.6 microgram/ml obtained by using polyclonal antibodies by the same ELISA. The whole virus particle was detected with this method to a limit of 2.3 x 10(2) PFU/ml. At the same time, an indirect ELISA was developed to detect ASFV antibodies. The results also indicate that this method may be a useful technique for epidemiological surveys.
开发了一种改进的使用单克隆抗体的固相酶联免疫吸附测定法（ELISA），用于检测猪样本中的非洲猪瘟病毒蛋白（VP73）。使用针对VP73的单克隆抗体可实现灵敏且特异的夹心ELISA。该测定法检测到VP-73的极限抗原浓度为0.05微克/毫升，低于通过相同ELISA使用多克隆抗体获得的0.6微克/毫升的检测限。用该方法检测完整病毒颗粒的极限为2.3×10² PFU/毫升。同时，开发了一种间接ELISA来检测非洲猪瘟病毒抗体。结果还表明，该方法可能是一种用于流行病学调查的有用技术。
