内源性基质辅助激光解吸电离傅里叶变换质谱中宽质荷比离子的捕获及其对基质用量的依赖性
Trapping of wide range mass-to-charge ions and dependence on matrix amount in internal source MALDI-FTMS.
作者信息
Jaber Arwah J, Kaufman Jacob, Liyanage Rohana, Akhmetova Eugenia, Marney Samuel, Wilkins Charles L
机构信息
Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, AR 72701, USA.
出版信息
J Am Soc Mass Spectrom. 2005 Nov;16(11):1772-80. doi: 10.1016/j.jasms.2005.06.014. Epub 2005 Sep 22.
Internal ionization source MALDI-FTMS shows clear variation of number average molecular weight (M(n)) for an equimolar mixture of four PEG polymers (PEG 2000, PEG 4000, PEG 6000, PEG 8000) when the 2,5-dihydroxy benzoic acid (DHB) matrix to PEG ratio is varied or the laser power is changed. As the matrix to analyte ratio is increased, the analyte signal of higher molecular mass ions increases and the signal of lower mass ions decreases. Laser power dependence studies show a similar trend. Possible explanations for these observations are discussed.
内电离源基质辅助激光解吸电离傅里叶变换质谱（MALDI-FTMS）显示，当2,5-二羟基苯甲酸（DHB）基质与聚乙二醇（PEG）聚合物的比例变化或激光功率改变时，四种聚乙二醇聚合物（PEG 2000、PEG 4000、PEG 6000、PEG 8000）的等摩尔混合物的数均分子量（M(n)）会出现明显变化。随着基质与分析物比例的增加，较高分子量离子的分析物信号增加，而较低质量离子的信号则降低。激光功率依赖性研究显示出类似的趋势。文中讨论了对这些观察结果的可能解释。
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