器官灌注与保存的解决方案：血液流变学方面

Solutions for organ perfusion and storage: haemorheologic aspects.

作者信息

Panzera P, Rotelli M T, Salerno A M, Cicco G, Catalano G, D'Elia G, Greco L, Lupo L, Memeo V

机构信息

Department of General Surgery and Liver Transplantation, University of Bari, Bari, Italy.

出版信息

Transplant Proc. 2005 Jul-Aug;37(6):2456-8. doi: 10.1016/j.transproceed.2005.06.024.

DOI:10.1016/j.transproceed.2005.06.024

PMID:16182707

Abstract

BACKGROUND

The hydroxyethyl starch (HES) contained in University of Wisconsin (UW) solution causes erythrocyte aggregation. The effect of UW on red blood cell (RBC) deformability is still unclear. HES-free preservation solutions, Celsior (CS) and Custodiol (CU) are available. In this study we evaluated whether they really showed a reduced aggregating and stiffening effect on RBCs when compared with UW. We was also evaluated the effect of these solutions on cellular membranes by measuring acetylcholinesterase (AChE), which is a marker of RBC membrane integrity.

METHODS

The determination of RBC aggregation and deformability was performed by a laser-assisted optical rotation cell analyzer (LORCA). AChE measurement was performed with a spectrophotometric technique.

RESULTS

The mean RBC aggregation index (AI) measured in pure blood control samples was 28.00 +/- 0.73%. The AI measured samples containing UW was 38.82 +/- 1.58%. In samples with CS, it was 13.307 +/- 0.64% and in samples with CU the mean AI was 12.47 +/- 0.42%. Also the RBC aggregating time was quicker in presence of UW compared with controls. AChE concentration in blood was 3.043 +/- 0.4 nmol. CS and UW did not produce any significant change; a significant reduction was found when CU was added to blood, namely 1.975 +/- 0.1 nmol (P < .05). The use of UW or CS or CU did not result in any significant change in RBC deformability.

DISCUSSION

CS and CU solutions do not aggregate erythrocytes, whereas Wisconsin does massively. CU causes an alteration of RBC cellular membrane as demonstrated by depletion of AChE.

摘要

背景

威斯康星大学（UW）溶液中含有的羟乙基淀粉（HES）会导致红细胞聚集。UW对红细胞（RBC）变形性的影响仍不清楚。不含HES的保存液，如Celsior（CS）和Custodiol（CU）已可获得。在本研究中，我们评估了与UW相比，它们是否真的对RBC显示出降低的聚集和僵化作用。我们还通过测量作为RBC膜完整性标志物的乙酰胆碱酯酶（AChE）来评估这些溶液对细胞膜的影响。

方法

通过激光辅助光学旋转细胞分析仪（LORCA）测定RBC聚集和变形性。用分光光度技术进行AChE测量。

结果

在纯血对照样品中测得的平均RBC聚集指数（AI）为28.00±0.73%。在含有UW的样品中测得的AI为38.82±1.58%。在含有CS的样品中，为13.307±0.64%，在含有CU的样品中，平均AI为12.47±0.42%。与对照相比，在存在UW的情况下RBC聚集时间也更快。血液中的AChE浓度为3.043±0.4 nmol。CS和UW未产生任何显著变化；当向血液中添加CU时发现显著降低，即1.975±0.1 nmol（P<.05）。使用UW或CS或CU不会导致RBC变形性有任何显著变化。