Simmet T, Pritze S, Thelen K I, Peskar B A
Department of Pharmacology and Toxicology, Ruhr-University, Bochum, F.R.G.
Eur J Pharmacol. 1992 Feb 18;211(3):319-22. doi: 10.1016/0014-2999(92)90387-j.
Rats injected intravenously with oleic acid developed pulmonary edema leading to hypoxia and hypercarbia. These changes were accompanied by an increase in immunoreactive endothelin (ir-ET) in plasma as early as 15 min after injection. At 45 min after injection plasma levels peaked at 114 +/- 19 pg/ml plasma (n = 8) and reached basal levels again after 240 min. In contrast, much larger amounts of ir-ET were found in the bronchoalveolar lavage fluid, with a peak at 120 min (2878 +/- 258 pg/lung, n = 7) preceding the maximum hypoxia observed at 180 min. In both plasma and bronchoalveolar lavage fluid samples ir-ET was characterized by reverse-phase HPLC as a mixture consisting mainly of ET-1 and smaller amounts of big ET-1, ET-2 and ET-3. In light of the biological effects of ET, the data suggest that these peptides might be of pathophysiological significance in this model of adult respiratory distress syndrome.
经静脉注射油酸的大鼠出现肺水肿，进而导致缺氧和高碳酸血症。早在注射后15分钟，这些变化就伴随着血浆中免疫反应性内皮素（ir-ET）的增加。注射后45分钟，血浆水平达到峰值，为114±19 pg/ml血浆（n = 8），并在240分钟后再次降至基础水平。相比之下，在支气管肺泡灌洗液中发现了大量得多的ir-ET，在120分钟时达到峰值（2878±258 pg/肺，n = 7），早于在180分钟时观察到的最大缺氧时间。在血浆和支气管肺泡灌洗液样本中，ir-ET通过反相高效液相色谱法被鉴定为主要由ET-1以及少量大ET-1、ET-2和ET-3组成的混合物。鉴于ET的生物学效应，这些数据表明这些肽在这种成人呼吸窘迫综合征模型中可能具有病理生理学意义。
