• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

On the catalytic mechanism of EcoRI and EcoRV. A detailed proposal based on biochemical results, structural data and molecular modelling.

作者信息

Jeltsch A, Alves J, Maass G, Pingoud A

机构信息

Institut für Biophysikalische Chemie, Medizinische Hochschule Hannover, Germany.

出版信息

FEBS Lett. 1992 Jun 8;304(1):4-8. doi: 10.1016/0014-5793(92)80576-3.

DOI:10.1016/0014-5793(92)80576-3
PMID:1618296
Abstract

EcoRI and EcoRV have a very similar active site, as is apparent from a comparison of the structures of their respective protein-DNA complexes. Based on structural and mechanistic data, as well as detailed molecular modelling presented here, a mechanism for the DNA cleavage by these enzymes is suggested in which the attacking water molecule is activated by the phosphate group 3' to the scissile phosphodiester bond, and in which the leaving group is protonated by a water molecule associated with the essential cofactor, Mg2+. The mechanism proposed may also apply to other nucleases.

摘要

相似文献

1
On the catalytic mechanism of EcoRI and EcoRV. A detailed proposal based on biochemical results, structural data and molecular modelling.
FEBS Lett. 1992 Jun 8;304(1):4-8. doi: 10.1016/0014-5793(92)80576-3.
2
Substrate-assisted catalysis in the cleavage of DNA by the EcoRI and EcoRV restriction enzymes.EcoRI和EcoRV限制酶切割DNA过程中的底物辅助催化作用。
Proc Natl Acad Sci U S A. 1993 Sep 15;90(18):8499-503. doi: 10.1073/pnas.90.18.8499.
3
Divalent metal ions at the active sites of the EcoRV and EcoRI restriction endonucleases.大肠杆菌限制性内切酶EcoRV和EcoRI活性位点处的二价金属离子
Biochemistry. 1995 Jan 17;34(2):697-704. doi: 10.1021/bi00002a037.
4
Structure-based redesign of the catalytic/metal binding site of Cfr10I restriction endonuclease reveals importance of spatial rather than sequence conservation of active centre residues.基于结构对Cfr10I限制性内切酶催化/金属结合位点进行重新设计，揭示了活性中心残基空间保守而非序列保守的重要性。
J Mol Biol. 1998 Jun 5;279(2):473-81. doi: 10.1006/jmbi.1998.1803.
5
Recognition and cleavage of DNA by type-II restriction endonucleases.II型限制性核酸内切酶对DNA的识别与切割
Eur J Biochem. 1997 May 15;246(1):1-22. doi: 10.1111/j.1432-1033.1997.t01-6-00001.x.
6
Specific binding by EcoRV endonuclease to its DNA recognition site GATATC.EcoRV核酸内切酶与它的DNA识别位点GATATC的特异性结合。
J Mol Biol. 1997 May 30;269(1):82-101. doi: 10.1006/jmbi.1997.1027.
7
Structural, functional, and evolutionary relationships between lambda-exonuclease and the type II restriction endonucleases.λ核酸外切酶与II型限制性内切核酸酶之间的结构、功能及进化关系。
Proc Natl Acad Sci U S A. 1998 Jul 7;95(14):7893-7. doi: 10.1073/pnas.95.14.7893.
8
A site-directed mutagenesis study to identify amino acid residues involved in the catalytic function of the restriction endonuclease EcoRV.一项用于鉴定参与限制性内切酶EcoRV催化功能的氨基酸残基的定点诱变研究。
Biochemistry. 1992 May 26;31(20):4808-15. doi: 10.1021/bi00135a010.
9
Five-stranded beta-sheet sandwiched with two alpha-helices: a structural link between restriction endonucleases EcoRI and EcoRV.由两条α-螺旋夹着的五链β-折叠：限制性内切酶EcoRI和EcoRV之间的结构联系。
Proteins. 1994 Nov;20(3):279-82. doi: 10.1002/prot.340200308.
10
Non-cognate enzyme-DNA complex: structural and kinetic analysis of EcoRV endonuclease bound to the EcoRI recognition site GAATTC.非同源酶 - DNA 复合物：与 EcoRI 识别位点 GAATTC 结合的 EcoRV 内切核酸酶的结构和动力学分析
J Mol Biol. 2005 Nov 18;354(1):121-36. doi: 10.1016/j.jmb.2005.09.046. Epub 2005 Oct 3.

引用本文的文献

1
Expression and purification of the modification-dependent restriction enzyme BisI and its homologous enzymes.修饰依赖性限制酶BisI及其同源酶的表达与纯化
Sci Rep. 2016 Jun 29;6:28579. doi: 10.1038/srep28579.
2
Engineering BspQI nicking enzymes and application of N.BspQI in DNA labeling and production of single-strand DNA.工程化BspQI切口酶及N.BspQI在DNA标记和单链DNA生产中的应用。
Protein Expr Purif. 2010 Feb;69(2):226-34. doi: 10.1016/j.pep.2009.09.003. Epub 2009 Sep 9.
3
Two crystal forms of the restriction enzyme MspI-DNA complex show the same novel structure.
限制性内切酶MspI-DNA复合物的两种晶体形式呈现出相同的新颖结构。
Protein Sci. 2005 Oct;14(10):2590-600. doi: 10.1110/ps.051565105.
4
KpnI restriction endonuclease and methyltransferase exhibit contrasting mode of sequence recognition.KpnI限制性内切酶和甲基转移酶表现出截然不同的序列识别模式。
Nucleic Acids Res. 2004 Jun 10;32(10):3148-55. doi: 10.1093/nar/gkh638. Print 2004.
5
The structural basis of damaged DNA recognition and endonucleolytic cleavage for very short patch repair endonuclease.极短补丁修复核酸内切酶识别受损DNA及进行核酸内切酶切割的结构基础。
Nucleic Acids Res. 2001 Sep 15;29(18):3775-83. doi: 10.1093/nar/29.18.3775.
6
Structure and function of type II restriction endonucleases.II型限制性核酸内切酶的结构与功能。
Nucleic Acids Res. 2001 Sep 15;29(18):3705-27. doi: 10.1093/nar/29.18.3705.
7
Substrate-assisted catalysis: molecular basis and biological significance.底物辅助催化：分子基础与生物学意义
Protein Sci. 2000 Jan;9(1):1-9. doi: 10.1110/ps.9.1.1.
8
Metal ion-mediated substrate-assisted catalysis in type II restriction endonucleases.II型限制性内切核酸酶中金属离子介导的底物辅助催化作用。
Proc Natl Acad Sci U S A. 1998 Nov 10;95(23):13489-94. doi: 10.1073/pnas.95.23.13489.
9
Cleavage properties of an oligonucleotide containing a bridged internucleotide 5'-phosphorothioate RNA linkage.
Nucleic Acids Res. 1995 Dec 11;23(23):4753-60. doi: 10.1093/nar/23.23.4753.
10
The crystal structure of EcoRV endonuclease and of its complexes with cognate and non-cognate DNA fragments.EcoRV核酸内切酶及其与同源和非同源DNA片段复合物的晶体结构。
EMBO J. 1993 May;12(5):1781-95. doi: 10.2210/pdb4rve/pdb.