Shentu Jingling, Wu Jianmin, Song Weihua, Jia Zhishen
Department of Chemistry, Zhejiang University, ZheDa Road 38, Hangzhou, Zhejiang 310027, China.
Int J Biol Macromol. 2005 Oct 30;37(1-2):42-6. doi: 10.1016/j.ijbiomac.2005.08.004. Epub 2005 Sep 23.
Chitosan microsphere (CS) was prepared by phase-inversion method as the support matrices. Cibacron Blue F3GA (CB) was covalently attached to the chitosan microspheres, and thus the novel dye-affinity adsorbent was obtained. These Cibacron Blue F3GA-attached chitosan microspheres (CB-CS) were used in the catalase (CAT) adsorption studies. The maximum CAT adsorption capacity of Cibacron Blue F3GA-attached chitosan microspheres was 28.4 mg/g at pH 7.0. Langmuir adsorption model was found to be applicable in interpreting CAT adsorption. Significant amount of the adsorbed CAT (up to 90.6%) was eluted in the elution medium containing 0.5 M NaSCN at pH 8.0. It appears that CB-CS can be applied for adsorption of CAT without causing any denaturation.
采用相转化法制备壳聚糖微球（CS）作为载体基质。将汽巴克隆蓝F3GA（CB）共价连接到壳聚糖微球上，从而获得新型染料亲和吸附剂。这些连接了汽巴克隆蓝F3GA的壳聚糖微球（CB-CS）用于过氧化氢酶（CAT）的吸附研究。在pH 7.0时，连接了汽巴克隆蓝F3GA的壳聚糖微球对CAT的最大吸附容量为28.4 mg/g。发现朗缪尔吸附模型适用于解释CAT的吸附。在pH 8.0含有0.5 M硫氰酸钠的洗脱介质中，大量吸附的CAT（高达90.6%）被洗脱。似乎CB-CS可用于吸附CAT而不会引起任何变性。
