壳聚糖微球作为固定化染料亲和载体用于过氧化氢酶吸附
Chitosan microspheres as immobilized dye affinity support for catalase adsorption.
作者信息
Shentu Jingling, Wu Jianmin, Song Weihua, Jia Zhishen
机构信息
Department of Chemistry, Zhejiang University, ZheDa Road 38, Hangzhou, Zhejiang 310027, China.
出版信息
Int J Biol Macromol. 2005 Oct 30;37(1-2):42-6. doi: 10.1016/j.ijbiomac.2005.08.004. Epub 2005 Sep 23.
Chitosan microsphere (CS) was prepared by phase-inversion method as the support matrices. Cibacron Blue F3GA (CB) was covalently attached to the chitosan microspheres, and thus the novel dye-affinity adsorbent was obtained. These Cibacron Blue F3GA-attached chitosan microspheres (CB-CS) were used in the catalase (CAT) adsorption studies. The maximum CAT adsorption capacity of Cibacron Blue F3GA-attached chitosan microspheres was 28.4 mg/g at pH 7.0. Langmuir adsorption model was found to be applicable in interpreting CAT adsorption. Significant amount of the adsorbed CAT (up to 90.6%) was eluted in the elution medium containing 0.5 M NaSCN at pH 8.0. It appears that CB-CS can be applied for adsorption of CAT without causing any denaturation.
采用相转化法制备壳聚糖微球（CS）作为载体基质。将汽巴克隆蓝F3GA（CB）共价连接到壳聚糖微球上，从而获得新型染料亲和吸附剂。这些连接了汽巴克隆蓝F3GA的壳聚糖微球（CB-CS）用于过氧化氢酶（CAT）的吸附研究。在pH 7.0时，连接了汽巴克隆蓝F3GA的壳聚糖微球对CAT的最大吸附容量为28.4 mg/g。发现朗缪尔吸附模型适用于解释CAT的吸附。在pH 8.0含有0.5 M硫氰酸钠的洗脱介质中，大量吸附的CAT（高达90.6%）被洗脱。似乎CB-CS可用于吸附CAT而不会引起任何变性。
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