Department of Chemistry, The University of Texas at San Antonio, One UTSA Circle, San Antonio, Texas 78249, United States.
Langmuir. 2010 Nov 16;26(22):17178-83. doi: 10.1021/la103035n. Epub 2010 Oct 14.
The adsorption conditions used to immobilize catalase onto thin films of carbon nanotubes were investigated to elucidate the conditions that produced films with maximum amounts of active catalase. The adsorption kinetics were monitored by spectroscopic ellipsometry, and the immobilized catalase films were then assayed for catalytic activity. The development of a volumetric optical model used to interpret the ellipsometric data is discussed. According to the results herein discussed, not only the adsorbed amount but also the initial adsorption rates determine the final catalytic activity of the adsorbed layer. The results described in this paper have direct implications on the rational design and analytical performance of enzymatic biosensors.
研究了将过氧化氢酶固定在碳纳米管薄膜上的吸附条件，以阐明产生具有最大过氧化氢酶活性的薄膜的条件。通过光谱椭圆术监测吸附动力学，然后对固定化过氧化氢酶薄膜进行催化活性测定。讨论了用于解释椭圆术数据的体积光学模型的开发。根据本文讨论的结果，不仅吸附量而且初始吸附速率决定了吸附层的最终催化活性。本文所述的结果对酶生物传感器的合理设计和分析性能具有直接影响。
