Babenko Nataliya A, Shakhova Elena G
Institute of Biology, Kharkov Karazin National University, 4, Svobody pl., Kharkov 61077, Ukraine.
Exp Gerontol. 2006 Jan;41(1):32-9. doi: 10.1016/j.exger.2005.08.008. Epub 2005 Sep 23.
The increased sphingolipid turnover in the liver is associated with elevation of free radical production and state of chronic inflammation at old age. Plant polyphenols are reported to exhibit antioxidant and anti-inflammatory effects. In the present paper, the lipids contents and ceramide production in the liver and hepatocytes as well as the correction of sphingolipid metabolism at old age using the mixture of Chamomilla recutita flavonoids (chamiloflan) or apigenin-7-glucoside or luteolin-7-glucoside alone have been investigated. To study the sphingolipids turnover, the [14C]serine-pre-labeled hepatocytes and [14C-methyl]- or [14C]palmitate-pre-labeled sphingomyelin (SM) and ceramide were used. The ceramide content was higher in the liver and hepatocytes of 24- and 27-28-month-old animals as compared to adult 3-month-old Wistar rats. An addition of flavonoids to the culture medium did not influence significantly on the lipids contents and metabolism in the isolated hepatocytes. The administration of flavonoids to old rats decreased the elevated neutral and acid SMases activities and ceramide mass and did not affect both the lipid content in the liver of adult animals and ceramide conversion to the sphingosine or SM. These results suggest that the SMases play a key role in the flavonoid-induced decrease of ceramide levels in the liver of old rats.
肝脏中鞘脂周转率的增加与老年时自由基产生的增加和慢性炎症状态有关。据报道，植物多酚具有抗氧化和抗炎作用。在本文中，研究了肝脏和肝细胞中的脂质含量和神经酰胺生成，以及使用洋甘菊黄酮混合物（chamiloflan）或单独的芹菜素 -7- 葡萄糖苷或木犀草素 -7- 葡萄糖苷对老年鞘脂代谢的校正作用。为了研究鞘脂周转率，使用了 [14C] 丝氨酸预标记的肝细胞以及 [14C-甲基] - 或 [14C] 棕榈酸预标记的鞘磷脂（SM）和神经酰胺。与成年 3 个月大的Wistar大鼠相比，24 个月和 27 - 28 个月大动物的肝脏和肝细胞中的神经酰胺含量更高。向培养基中添加黄酮类化合物对分离的肝细胞中的脂质含量和代谢没有显著影响。给老年大鼠施用黄酮类化合物可降低升高的中性和酸性鞘磷脂酶活性以及神经酰胺量，并且对成年动物肝脏中的脂质含量和神经酰胺向鞘氨醇或鞘磷脂的转化均无影响。这些结果表明，鞘磷脂酶在黄酮类化合物诱导的老年大鼠肝脏中神经酰胺水平降低中起关键作用。
