甘菊：一种抗炎剂，通过阻断 RelA/p65 活性抑制诱导型一氧化氮合酶的表达。
Chamomile: an anti-inflammatory agent inhibits inducible nitric oxide synthase expression by blocking RelA/p65 activity.
机构信息
Department of Urology, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA.
出版信息
Int J Mol Med. 2010 Dec;26(6):935-40. doi: 10.3892/ijmm_00000545.
Abstract
Chamomile has long been used in traditional medicine for the treatment of inflammation-related disorders. In this study we investigated the inhibitory effects of chamomile on nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) expression, and explored its potential anti-inflammatory mechanisms using RAW 264.7 macrophages. Chamomile treatment inhibited LPS-induced NO production and significantly blocked IL-1β, IL-6 and TNFα-induced NO levels in RAW 264.7 macrophages. Chamomile caused reduction in LPS-induced iNOS mRNA and protein expression. In RAW 264.7 macrophages, LPS-induced DNA binding activity of RelA/p65 was significantly inhibited by chamomile, an effect that was mediated through the inhibition of IKKβ, the upstream kinase regulating NF-κB/Rel activity, and degradation of inhibitory factor-κB. These results demonstrate that chamomile inhibits NO production and iNOS gene expression by inhibiting RelA/p65 activation and supports the utilization of chamomile as an effective anti-inflammatory agent.
摘要
洋甘菊在传统医学中一直被用于治疗与炎症相关的疾病。在这项研究中，我们研究了洋甘菊对一氧化氮（NO）产生和诱导型一氧化氮合酶（iNOS）表达的抑制作用，并利用 RAW 264.7 巨噬细胞探索了其潜在的抗炎机制。洋甘菊处理抑制 LPS 诱导的 NO 产生，并显著阻断 RAW 264.7 巨噬细胞中 IL-1β、IL-6 和 TNFα诱导的 NO 水平。洋甘菊导致 LPS 诱导的 iNOS mRNA 和蛋白质表达减少。在 RAW 264.7 巨噬细胞中，洋甘菊显著抑制 LPS 诱导的 RelA/p65 的 DNA 结合活性，该作用是通过抑制 IKKβ 介导的，IKKβ 是调节 NF-κB/Rel 活性的上游激酶，以及抑制因子-κB 的降解。这些结果表明，洋甘菊通过抑制 RelA/p65 的激活来抑制 NO 的产生和 iNOS 基因的表达，支持将洋甘菊用作有效的抗炎剂。
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2
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Eur J Pharmacol. 2008 May 31;586(1-3):340-9. doi: 10.1016/j.ejphar.2008.02.044. Epub 2008 Feb 26.
3
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6
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