Farzin Davood, Mansouri Nazanin
Department of Pharmacology, School of Medicine, Mazandaran University of Medical Sciences, Sari 48168, Iran.
Eur Neuropsychopharmacol. 2006 Jul;16(5):324-8. doi: 10.1016/j.euroneuro.2005.08.005. Epub 2005 Sep 22.
The purpose of the present study was to determine the effects of harmane, norharmane and harmine on the immobility time in the mouse forced swim test (FST) - an animal model of depression. After 30 min of the beta-carbolines injections, mice were placed individually in a vertical glass cylinder (height, 25 cm; diameter, 12 cm) containing water about 15 cm deep at 22+/-1 degrees C and forced to swim. Treatment of animals with harmane (5-15 mg/kg, i.p.), norharmane (2.5-10 mg/kg, i.p.) and harmine (5-15 mg/kg, i.p.) reduced dose-dependently the time of immobility. Their antidepressant-like effects were not affected by pretreatment with reserpine at the dose of 5 mg/kg, i.p., 18 h before the test, which did not modify the immobility time. Conversely, when flumazenil (5 mg/kg, i.p.) was administered 30 min before the test, it was able to antagonize completely the antidepressant-like effects of harmane, norharmane and harmine. It was concluded that harmane, norharmane and harmine reduce the immobility time in this test, suggesting an antidepressant-like effect, via an inverse-agonistic mechanism located in the benzodiazepine receptors.
本研究的目的是确定去氢骆驼蓬碱、去甲去氢骆驼蓬碱和骆驼蓬碱对小鼠强迫游泳试验（FST）——一种抑郁动物模型——中不动时间的影响。在注射β-咔啉30分钟后，将小鼠单独放入一个垂直的玻璃圆筒（高25厘米；直径12厘米）中，圆筒内装有深度约15厘米的水，水温为22±1摄氏度，使其被迫游泳。用去氢骆驼蓬碱（5 - 15毫克/千克，腹腔注射）、去甲去氢骆驼蓬碱（2.5 - 10毫克/千克，腹腔注射）和骆驼蓬碱（5 - 15毫克/千克，腹腔注射）处理动物，可剂量依赖性地减少不动时间。在试验前18小时腹腔注射5毫克/千克利血平进行预处理，对它们的抗抑郁样作用没有影响，利血平并未改变不动时间。相反，在试验前30分钟腹腔注射氟马西尼（5毫克/千克），它能够完全拮抗去氢骆驼蓬碱、去甲去氢骆驼蓬碱和骆驼蓬碱的抗抑郁样作用。得出的结论是，去氢骆驼蓬碱、去甲去氢骆驼蓬碱和骆驼蓬碱在该试验中可减少不动时间，提示通过位于苯二氮䓬受体的反向激动机制产生抗抑郁样作用。
