Ye Xiu-jin, Lin Mao-fang
Department of Hematology, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.
Zhonghua Xue Ye Xue Za Zhi. 2005 Jun;26(6):363-6.
To investigate the relationship between expression of vascular endothelial growth factor (VEGF) and pathogenesis of acute leukemia (AL).
VEGF mRNA expression was analysed by using semi-quantitative RT-PCR technique. VEGF level of culture supernatant of the bone marrow mononuclear cell (BMMNC) was detected by ELISA. The influence of culture supernatant of the BMMNC on proliferation of human umbilical vein endothelial cell line huECV304 in vitro was determined by MTT assay.
The median VEGF/beta-actin expression level of BMMNC in newly diagnosed untreated AL group was higher (0.86) than that in remission group (0.41) and normal control group (0.39) (P < 0.01, both), but was no difference from the relapse group (1.02, P > 0.05). Among the patients with newly diagnosed untreated AL, VEGF/beta-actin level in AML (1.03) was statistically higher than that in ALL (0.61) (P < 0.05). The VEGF level in 72 h culture supernatant of BMMNC was higher in newly diagnosed untreated AL group (91.48 ng/L) than in remission group (31.91 ng/L) and normal control group (28.71 ng/L) (P < 0.01). In ALL group (32.76 ng/L), the VEGF level of 72 h culture supernatant was much lower than that in AML group (173.49 ng/L) (P < 0.01). The proliferation of huECV304 cells after co-cultured with the 72 h culture supernatant of AML BMMNC with addition of anti-VEGF antibody was inhibited notably compared with culture supernatant alone (P < 0.01). However, no such result was found in ALL group (P > 0.05).
The mRNA expression and secretion of VEGF in AML cells were higher than those in ALL cells. The regulators that participated angiogenesis were different in ALL and AML patients.
探讨血管内皮生长因子（VEGF）表达与急性白血病（AL）发病机制之间的关系。
采用半定量逆转录聚合酶链反应（RT-PCR）技术分析VEGF mRNA表达。采用酶联免疫吸附测定（ELISA）法检测骨髓单个核细胞（BMMNC）培养上清液中的VEGF水平。采用MTT法测定BMMNC培养上清液对人脐静脉内皮细胞系huECV304体外增殖的影响。
初诊未治疗的AL组BMMNC中VEGF/β-肌动蛋白表达水平中位数（0.86）高于缓解组（0.41）和正常对照组（0.39）（均P<0.01），但与复发组（1.02）无差异（P>0.05）。初诊未治疗的AL患者中，急性髓系白血病（AML）患者的VEGF/β-肌动蛋白水平（1.03）高于急性淋巴细胞白血病（ALL）患者（0.61）（P<0.05）。初诊未治疗的AL组BMMNC 72小时培养上清液中的VEGF水平（91.48 ng/L）高于缓解组（31.91 ng/L）和正常对照组（28.71 ng/L）（P<0.01）。ALL组72小时培养上清液中的VEGF水平（32.76 ng/L）明显低于AML组（173.49 ng/L）（P<0.01）。与单独培养上清液相比，AML的BMMNC 72小时培养上清液添加抗VEGF抗体后与huECV304细胞共培养后，huECV304细胞的增殖受到显著抑制（P<0.01）。然而，ALL组未发现此结果（P>0.05）。
AML细胞中VEGF的mRNA表达和分泌高于ALL细胞。ALL和AML患者中参与血管生成的调节因子不同。
