Santos-Filho Sebastião David, Bernardo-Filho Mario
Programa de Pós-Graduação em Ciências da Saúde da Universidade Federal do Rio Grande do Norte.
Acta Cir Bras. 2005;20 Suppl 1:121-5.
To evaluate the effect of a hiperico extract (Hypericum perforatum) on the labeling of blood elements with technetium-99m (99mTc) and in the bioavailability of the radiopharmaceutical sodium pertechnetate in Wistar rats.
Blood (heparinized) withdrawn from Wistar rats is incubated with a hiperico extract, with a stannous cloride and with 99mTc, as sodium pertechnetate (99mTcONa). Plasma (P) and cells (C) are isolated by centrifugation. Samples of P and C are also precipitated with trichloroacetic acid (TCA 5%) and soluble (FS-P; FS-C) and isoluble (FI-P; FI-C) fractions are separated. In the bioavailability analysis, the extract or NaCl 0.9% solution is administrated into Wistar rats (gavage) during 15 days. Sodium pertechnetate was administered and after 10 min, the animals are sacrificed, the organs were isolated, the radioactivity determined in a well counter, and the percentages of radioactivity per gram (%ATI/g) in the organs are calculated.
The hiperico extract decreasedsignificantly (P < 0.05) the %ATI in the cells, cellular insoluble fraction and plasma insoluble fraction. The biodistribution was significantly (P < 0.01) decreased in bone, muscle and thyroid and significantly (P < 0.05) increased in pancreas.
The analysis of the results indicates that in studied extract should have substances that should oxidize the stannous ion, reducing the fixation of the 99mTc on the erythrocytes and plasma and cellular proteins. Moreover, it could produce metabolic alterations with influence in the uptake of the radiopharmaceutical 99mTcO4Na in bone, muscle, pancreas and thyroid.
评估金丝桃提取物（贯叶连翘）对用锝-99m（99mTc）标记血液成分以及放射性药物高锝酸钠在Wistar大鼠体内生物利用度的影响。
从Wistar大鼠采集的血液（肝素化）与金丝桃提取物、氯化亚锡以及作为高锝酸钠（99mTcONa）的99mTc一起孵育。通过离心分离血浆（P）和细胞（C）。P和C的样本也用三氯乙酸（5% TCA）沉淀，并分离出可溶性（FS-P；FS-C）和不溶性（FI-P；FI-C）部分。在生物利用度分析中，提取物或0.9%氯化钠溶液在15天内给Wistar大鼠灌胃。给予高锝酸钠，10分钟后处死动物，分离器官，在井型计数器中测定放射性，并计算器官中每克放射性百分比（%ATI/g）。
金丝桃提取物显著降低了细胞、细胞不溶性部分和血浆不溶性部分的%ATI（P < 0.05）。骨、肌肉和甲状腺中的生物分布显著降低（P < 0.01），胰腺中的生物分布显著增加（P < 0.05）。
结果分析表明，所研究的提取物中应含有能氧化亚锡离子的物质，减少99mTc在红细胞、血浆和细胞蛋白上的固定。此外，它可能会引起代谢改变，影响放射性药物99mTcO4Na在骨、肌肉、胰腺和甲状腺中的摄取。
