Amorim L F, Catanho M T J A, Terra D A, Brandão K C, Holanda C M C X, Jales-Júnior L H, Brito L M L, Gomes M L, De Melo V G B, Bernardo-Filho M, Cavalcanti Jales R L
Universidade Federal do Rio Grande do Norte, Centro de Biociências, Depto de Fisiologia. Av. Salgado Filho, 3000, Natal, RN 59078-970, Brazil.
Cell Mol Biol (Noisy-le-grand). 2003 Jun;49(4):501-7.
The many desirable characteristics of technetium-99m (99mTc) have stimulated the development of labeling techniques for different molecular and cellular structures. It is generally accepted that a variety of factors other than disease can alter the bioavailability of radiopharmaceuticals and one such factor is the drug therapy. The use of medicinal plants has increased in the last decades all over the world. Punica granatum (pomegranata) is used as food or as medication in folk medicine for antiviral, anthelmintic, antifungal, antibacterial and antimicrobial activity. We have studied in rats, the effect of the medicinal plant Punica granatum on the bioavailability of the radiopharmaceutical 99mTc-sodium pertechnetate (Na(99m)TcO4). The infusion of pomegranata was administered by intragastric via into Wistar rats during seven days. After that, the animals received by ocular plexus via, 0.1 ml of the Na(99m)TcO4 (3.7MBq) and the animals were rapidly sacrificed after 5, 20 and 40 min. The organs were isolated (brain, heart, thyroid, liver, lungs, kidneys, stomach, testis, intestines, pancreas, spleen, bladder, muscle and bone), the radioactivity determined in a well counter, the percentages of radioactivity (%ATI) in the organs were calculated and statistical analyses were performed by Wilcoxon test (p < 0.05). The results have shown a significant (p < 0.05) increase of the activity of the Na(99m)TcO4 in spleen, heart, stomach, liver, stout bowel, pancreas, lungs and testis at 5 min. Twenty minutes after the administration of the radiopharmaceutical, the analysis of the results reveals a significant (p < 0.05) increase of the %ATI in heart, stomach, femur, pancreas, lungs and kidneys. Forty minutes after the administration of the Na(99m)TcO4, the results show a significant (p < 0.05) increase in spleen, brain, heart, stomach, liver, stout bowel, muscle, femur, lungs, pancreas, kidneys and testis. These results can be justified by therapeutic effect of this extract and/or by generation of active metabolites capable to interfere with the biodistribution of the studied radiopharmaceutical.
锝-99m（99mTc）的诸多理想特性推动了针对不同分子和细胞结构的标记技术的发展。人们普遍认为，除疾病外，多种因素会改变放射性药物的生物利用度，药物治疗就是其中一个因素。在过去几十年里，世界各地对药用植物的使用有所增加。石榴（Punica granatum）在民间医学中用作食物或药物，具有抗病毒、驱虫、抗真菌、抗菌和抗微生物活性。我们在大鼠身上研究了药用植物石榴对放射性药物高锝酸钠（Na(99m)TcO4）生物利用度的影响。连续七天通过胃内途径向Wistar大鼠输注石榴提取物。之后，通过眼丛向动物注射0.1 ml的Na(99m)TcO4（3.7MBq），并在5分钟、20分钟和40分钟后迅速处死动物。分离出器官（脑、心脏、甲状腺、肝脏、肺、肾脏、胃、睾丸、肠道、胰腺、脾脏、膀胱、肌肉和骨骼），在井型计数器中测定放射性，计算器官中放射性百分比（%ATI），并通过Wilcoxon检验进行统计分析（p < 0.05）。结果显示，在5分钟时，脾脏、心脏、胃、肝脏、粗肠、胰腺、肺和睾丸中Na(99m)TcO4的活性显著（p < 0.05）增加。在给予放射性药物20分钟后，结果分析显示心脏、胃、股骨、胰腺、肺和肾脏中的%ATI显著（p < 0.05）增加。在给予Na(99m)TcO4 40分钟后，结果显示脾脏、脑、心脏、胃、肝脏、粗肠、肌肉、股骨、肺、胰腺、肾脏和睾丸中的放射性显著（p < 0.05）增加。这些结果可以通过该提取物的治疗效果和/或能够干扰所研究放射性药物生物分布的活性代谢产物的产生来解释。
