用感应耦合等离子体原子发射光谱仪测量外淋巴中的金属元素。

Metallic elements in the perilymph measured with an inductively-coupled plasma atomic emission spectrometer.

作者信息

Hara A, Senarita M, Komeno M, Kusakari J

机构信息

Department of Otorhinolaryngology, University of Tsukuba, Japan.

出版信息

Hear Res. 1992 May;59(2):117-20. doi: 10.1016/0378-5955(92)90108-y.

DOI:10.1016/0378-5955(92)90108-y

PMID:1618704

Abstract

Metallic elements in the perilymph of the scala tympani in normal and acoustically overstimulated guinea pigs were measured using a new method, an inductively-coupled plasma atomic emission spectrometry. The concentrations of phosphorus and eight metallic elements, i.e. calcium, copper, iron, potassium, magnesium, sodium, lead and zinc were measured simultaneously in a 2 microliters sample of perilymph. The mean concentration values of calcium, copper, iron, magnesium, phosphorus, lead and zinc were 2.03 mM, 38.5 microM, 69.3 microM, 0.822 mM, 0.851 mM, 43.5 microM and 25.0 microM, respectively. There was no significant effect of acoustic overstimulation on the concentrations of these elements except for magnesium, which decreased significantly after the exposure to a intense sound (2 kHz, 115 dB SPL) for 15 min. This is the first report describing the synchronous determination of metallic poly-elements, including copper, iron, lead and zinc, in the perilymph.

摘要

采用一种新方法——电感耦合等离子体原子发射光谱法，对正常和声学过度刺激的豚鼠鼓阶外淋巴中的金属元素进行了测量。在2微升外淋巴样本中同时测量了磷和八种金属元素，即钙、铜、铁、钾、镁、钠、铅和锌的浓度。钙、铜、铁、镁、磷、铅和锌的平均浓度值分别为2.03 mM、38.5 microM、69.3 microM、0.822 mM、0.851 mM、43.5 microM和25.0 microM。除镁外，声学过度刺激对这些元素的浓度没有显著影响，暴露于强度为2 kHz、115 dB SPL的强声15分钟后，镁的浓度显著降低。这是首次描述在外淋巴中同步测定包括铜、铁、铅和锌在内的多种金属元素的报告。