Somogyi Anikó, Herold Magdolna, Kocsis Ibolya, Nagy Géza, Somfai Gábor, Studinger Péter
Semmelweis Egyetem, Altalános Orvostudományi Kar, Budapest, II. Belgyógyászati Klinika, Budapest.
Orv Hetil. 2005 Aug 28;146(35):1813-8.
The effect of supplementary Vitamin E on the vitamin content of lipoproteins in young men and women. Inappropriate vitamin and trace element supplementation may facilitate the development of atherosclerosis. It is known that Vitamin E protects lipids from oxidative stress, while clinical signs of atherosclerosis appear later in women compared to men.
(1) The increase of vitamin E in plasma and plasma lipoproteins after 4 weeks of supplementation vitamin E was investigated, (2) furthermore it was tested whether a proportion shift occurs in alpha-tocopherol content of lipoproteins, (3) and checked for gender-related differences in plasma and plasma lipoprotein vitamin E levels before, during and after treatment, (4) plasma CRP levels as a marker of lipid peroxidation were also followed.
5-5 young healthy men and women took part in the study, receiving 700 IU/day Vitamin E for one month. Each subject was studied before and at the end of treatment, and also one month after treatment. HDL and LDL-VLDL containing lipoproteins were separated. Vitamin E and hsCRP levels were measured (by HPLC and an immunoturbidimetric method, respectively).
Vitamin E treatment induced in both genders an approximately threefold increase in vitamin E concentration in HDL-cholesterol (8.1 +/- 1.7 micromol/l vs. 22.5 +/- 7.5 micromol/l, p < 0.001), and a twofold increase in LDL-VLDL-cholesterol (22.0 +/- 3.7 micromol/l vs. 49.0 +/- 9.0 micromol/l, p < 0.001). Plasma and HDL vitamin E levels were higher in women than in men at the onset of treatment (6.8 +/- 0.96 micromol/l vs. 9.5 +/- 1.10 micromol/l), but during the treatment these gender-related differences disappeared. When plasma vitamin E concentration were considered 100% and the changes of the vitamin E concentrations of lipoproteins were calculated, it was found that supplementation with vitamin E in men increased the vitamin E concentration of LDL-VLDL cholesterol to a higher extent compared to women (LDL-VLDL % in men: 59.8 +/- 7.43%, in women: 49.3 +/- 7.41%, p < 0.05). All the observed changes regressed one month after cessation of supplementation. The level of hsCRP decreased during vitamin E treatment (1.07 +/- 0.9 mg/l vs. 0.2 +/- 0.14 mg/l, p < 0.001), and remained suppressed after the cessation of treatment (0.37 +/- 0.4, p < 0.01).
These results support the hypothesis that women at young age are better protected against lipid-peroxidation as compared to men because of higher HDL vitamin E concentrations. Vitamin E supplementation in men eliminates this concentration difference between genders, and also increases LDL-VLDL vitamin E. In both genders high concentration of vitamin E in lipoproteins was associated with low hsCRP concentration.
补充维生素E对年轻男性和女性脂蛋白中维生素含量的影响。不恰当的维生素和微量元素补充可能会促进动脉粥样硬化的发展。已知维生素E可保护脂质免受氧化应激影响，且女性动脉粥样硬化的临床症状出现时间比男性晚。
（1）研究补充维生素E 4周后血浆和血浆脂蛋白中维生素E的增加情况，（2）此外，测试脂蛋白中α-生育酚含量是否发生比例变化，（3）检查治疗前、治疗期间和治疗后血浆和血浆脂蛋白维生素E水平的性别差异，（4）还跟踪血浆CRP水平作为脂质过氧化的标志物。
55名年轻健康男性和女性参与研究，每天服用700 IU维生素E，为期一个月。对每个受试者在治疗前、治疗结束时以及治疗后一个月进行研究。分离出含高密度脂蛋白（HDL）和低密度脂蛋白-极低密度脂蛋白（LDL-VLDL）的脂蛋白。分别通过高效液相色谱法（HPLC）和免疫比浊法测量维生素E和超敏CRP（hsCRP）水平。
维生素E治疗使男女两性HDL胆固醇中的维生素E浓度均增加约三倍（8.1±1.7微摩尔/升对22.5±7.5微摩尔/升，p<0.001），LDL-VLDL胆固醇中的维生素E浓度增加两倍（22.0±3.7微摩尔/升对49.0±9.0微摩尔/升，p<0.001）。治疗开始时女性的血浆和HDL维生素E水平高于男性（6.8±0.96微摩尔/升对9.5±1.10微摩尔/升），但在治疗期间这些性别差异消失。当将血浆维生素E浓度视为100%并计算脂蛋白中维生素E浓度的变化时，发现男性补充维生素E后LDL-VLDL胆固醇中维生素E浓度的增加幅度高于女性（男性LDL-VLDL的比例为59.8±7.43%，女性为49.3±7.41%，p<0.05）。补充停止一个月后，所有观察到的变化均恢复。维生素E治疗期间hsCRP水平下降（1.07±0.9毫克/升对0.2±0.14毫克/升，p<0.001），治疗停止后仍受抑制（0.37±0.4，p<0.01）。
这些结果支持以下假设，即年轻女性由于HDL维生素E浓度较高，比男性更能抵御脂质过氧化。男性补充维生素E消除了性别之间的这种浓度差异，同时也增加了LDL-VLDL中的维生素E。在男女两性中，脂蛋白中高浓度的维生素E与低hsCRP浓度相关。
