Sohn Kyung-Cheol, Do Su-Il
Ajou University, Department of Life Science, Laboratory of Functional Glycomics, Republic of Korea.
Biochem Biophys Res Commun. 2005 Nov 11;337(1):256-63. doi: 10.1016/j.bbrc.2005.09.049.
Zebrafish OGT (zOGT) sequence was identified in zebrafish (Danio rerio) genome and six different transcriptional variants of zOGT, designated var1 to var6, were isolated. Here we describe the developmental regulation of zOGT variants at transcriptional level and characterization of their OGT activities of protein O-GlcNAcylation. OGT transcriptional variants in zebrafish were differentially generated by alternative splicing and in particular, var1 and var2 were contained by 48 bp intron as a novel exon sequence, demonstrating that this form of OGT was not found in mammals. Transcript analysis revealed that var1 and var2 were highly expressed at early phase of development including unfertilized egg until dome stage whereas var3 and var4 were begins to be expressed at sphere stage until late phase of development. Our data indicate that var1 and var2 are likely to be maternal transcripts. The protein expression assay in Escherichia coli-p62 system showed that OGT activities of var3 and var4 were found to be only active whereas those of other variants were inactive.
斑马鱼OGT（zOGT）序列是在斑马鱼（Danio rerio）基因组中鉴定出来的，并且分离出了zOGT的六种不同转录变体，命名为var1至var6。在此，我们描述了zOGT变体在转录水平上的发育调控及其蛋白质O-糖基化修饰的OGT活性特征。斑马鱼中的OGT转录变体通过可变剪接差异产生，特别是var1和var2包含48 bp内含子作为新的外显子序列，这表明这种形式的OGT在哺乳动物中未被发现。转录分析显示，var1和var2在发育早期高度表达，包括未受精卵直至穹顶期，而var3和var4在球形期开始表达直至发育后期。我们的数据表明，var1和var2可能是母体转录本。在大肠杆菌-p62系统中的蛋白质表达分析表明，发现只有var3和var4的OGT活性具有活性，而其他变体的OGT活性无活性。
