Donizetti Aldo, Liccardo Daniela, Esposito Daniela, Del Gaudio Rosanna, Locascio Annamaria, Ferrara Diana, Minucci Sergio, Aniello Francesco
Department of Structural and Functional Biology, University of Naples Federico II, Napoli, Italy.
Dev Dyn. 2008 Apr;237(4):1112-8. doi: 10.1002/dvdy.21492.
We show that ptma, a single copy gene found in all organisms investigated so far, is duplicated in zebrafish. The two genes, ptmaa and ptmab, are individually controlled as indicated by their different expression patterns during embryonic development. Only the ptmab transcript is observed at 4 and 8 hpf of development in all embryonic cells, whereas both genes are expressed at later stages as revealed by in situ hybridization studies. In most cases, the two genes are expressed in the same territories, but only the ptmaa transcript was found in the trigeminal ganglion and in endodermal pouches. In the eye, at 72 hpf, the ptmaa and ptmab transcripts were found in amacrine cells, whereas only the ptmab transcript appeared in horizontal cells. The existence of two prothymosin genes indicates that their function in cell proliferation and differentiation is more complex in fishes than in mammals.
我们发现，ptma是迄今为止在所有已研究生物中均为单拷贝的基因，而在斑马鱼中该基因发生了复制。这两个基因，ptmaa和ptmab，如它们在胚胎发育过程中不同的表达模式所示，受到各自独立的调控。在发育4小时和8小时的所有胚胎细胞中，仅观察到ptmab转录本，而原位杂交研究显示，这两个基因在发育后期均有表达。在大多数情况下，这两个基因在相同区域表达，但仅在三叉神经节和内胚层囊中发现了ptmaa转录本。在眼睛中，在72小时胚胎期，ptmaa和ptmab转录本在无长突细胞中被发现，而仅ptmab转录本出现在水平细胞中。两个原胸腺素基因的存在表明，它们在细胞增殖和分化中的功能在鱼类中比在哺乳动物中更为复杂。
