Bódalo A, Gómez J L, Gómez E, Bastida J, Máximo M F
Chemical Engineering Department, University of Murcia, Campus de Espinardo, 30071 Murcia, Spain.
Chemosphere. 2006 Apr;63(4):626-32. doi: 10.1016/j.chemosphere.2005.08.007. Epub 2005 Sep 26.
This paper describes a comparison between horseradish peroxidase (HRP) and soybean peroxidase (SBP), the two most widely used commercial peroxidases for the removal of phenol from wastewater. Both enzymes achieve maximal removal efficiency in a neutral pH medium although they are still quite active in a pH range of between 6.0 and 8.0. The fact that both enzymes show similar phenol elimination levels at any temperature between 25 and 40 degrees C is also of interest. Studies were carried out in the absence and presence of different concentrations of a stabilisation additive, polyethylene glycol (PEG), in an attempt to optimise the phenol elimination procedure. The final choice of peroxidase will depend on the characteristics of the effluent, operational requirements and on economic considerations. Our findings show that HRP acts faster than SBP but is more susceptible to inactivation, although it is better protected by PEG. In consequence HRP will be the most suitable choice but the addition of a sufficient amount of PEG is critical.
本文描述了辣根过氧化物酶（HRP）和大豆过氧化物酶（SBP）之间的比较，这两种酶是废水脱酚中应用最广泛的商业过氧化物酶。尽管这两种酶在pH值6.0至8.0范围内仍具有相当高的活性，但它们在中性pH介质中均能达到最大去除效率。两种酶在25至40摄氏度之间的任何温度下均显示出相似的苯酚去除水平，这一事实也值得关注。研究在有无不同浓度的稳定添加剂聚乙二醇（PEG）的情况下进行，以优化苯酚去除程序。过氧化物酶的最终选择将取决于废水的特性、操作要求和经济因素。我们的研究结果表明，HRP比SBP作用更快，但更容易失活，尽管它受到PEG的更好保护。因此，HRP将是最合适的选择，但添加足够量的PEG至关重要。
