降低MA104细胞的胆固醇含量会抑制受体介导的叶酸转运。
Lowering the cholesterol content of MA104 cells inhibits receptor-mediated transport of folate.
作者信息
Chang W J, Rothberg K G, Kamen B A, Anderson R G
机构信息
Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas 75235.
出版信息
J Cell Biol. 1992 Jul;118(1):63-9. doi: 10.1083/jcb.118.1.63.
Abstract
The folate receptor is clustered on the surface of MA104 cells in association with caveolae. This relationship is thought to be essential for the proper internalization and recycling of the receptor during the delivery of 5-methyltetrahydrofolate to the cytoplasm of folate-depleted cells. Both the clustered organization of the receptor and the integrity of caveolae are disrupted when cells are deprived of cholesterol. We now show that cholesterol depletion of MA104 cells markedly reduces the rate of 5-methyltetrahydrofolate internalization and causes a 70% decline in the number of receptors present in the internal, recycling compartment. This effect is consistent with morphologic data showing that cholesterol-depleted MA104 cells have a reduced number of caveolae as well as fewer receptors per caveolae.
摘要
叶酸受体聚集在MA104细胞表面，与小窝相关联。这种关系被认为对于在将5-甲基四氢叶酸递送至叶酸缺乏细胞的细胞质过程中受体的正确内化和再循环至关重要。当细胞缺乏胆固醇时，受体的聚集组织和小窝的完整性都会受到破坏。我们现在表明，MA104细胞的胆固醇耗竭显著降低了5-甲基四氢叶酸的内化速率，并导致内部再循环区室中存在的受体数量下降70%。这种效应与形态学数据一致，该数据表明胆固醇耗竭的MA104细胞小窝数量减少，每个小窝中的受体也更少。
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