胆固醇控制着5-甲基四氢叶酸糖磷脂锚定膜受体的聚集。
Cholesterol controls the clustering of the glycophospholipid-anchored membrane receptor for 5-methyltetrahydrofolate.
作者信息
Rothberg K G, Ying Y S, Kamen B A, Anderson R G
机构信息
Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas 75235.
出版信息
J Cell Biol. 1990 Dec;111(6 Pt 2):2931-8. doi: 10.1083/jcb.111.6.2931.
Abstract
The folate receptor is a glycosyl-phosphatidylinositol (GPI)-anchored membrane protein that mediates the delivery of 5-methyltetrahydrofolate to the cytoplasm of MA104 cells. Ordinarily the receptor is sequestered into numerous discrete clusters that are associated with an uncoated pit membrane specialization called a caveola. By using two different methodological approaches, we found that the maintenance of both receptor clusters and caveolae depends upon the presence of cholesterol in the membrane. These results suggest that cholesterol plays a critical role in maintaining the caveola membrane domain and modulates the interaction of GPI-anchored membrane proteins via their phospholipid anchors.
摘要
叶酸受体是一种糖基磷脂酰肌醇（GPI）锚定的膜蛋白，它介导5-甲基四氢叶酸向MA104细胞胞质的转运。通常情况下，该受体被隔离在众多离散的簇中，这些簇与一种称为小窝的无包被凹陷膜特化结构相关。通过使用两种不同的方法，我们发现受体簇和小窝的维持都依赖于膜中胆固醇的存在。这些结果表明，胆固醇在维持小窝膜结构域中起关键作用，并通过其磷脂锚调节GPI锚定膜蛋白的相互作用。
相似文献
1
Cholesterol controls the clustering of the glycophospholipid-anchored membrane receptor for 5-methyltetrahydrofolate.胆固醇控制着5-甲基四氢叶酸糖磷脂锚定膜受体的聚集。
J Cell Biol. 1990 Dec;111(6 Pt 2):2931-8. doi: 10.1083/jcb.111.6.2931.
2
The glycophospholipid-linked folate receptor internalizes folate without entering the clathrin-coated pit endocytic pathway.糖磷脂连接的叶酸受体可内化叶酸，而不进入网格蛋白包被小窝的内吞途径。
J Cell Biol. 1990 Mar;110(3):637-49. doi: 10.1083/jcb.110.3.637.
3
Clustered folate receptors deliver 5-methyltetrahydrofolate to cytoplasm of MA104 cells.聚集的叶酸受体将5-甲基四氢叶酸输送到MA104细胞的细胞质中。
J Cell Biol. 1996 Sep;134(5):1169-77. doi: 10.1083/jcb.134.5.1169.
4
Lowering the cholesterol content of MA104 cells inhibits receptor-mediated transport of folate.降低MA104细胞的胆固醇含量会抑制受体介导的叶酸转运。
J Cell Biol. 1992 Jul;118(1):63-9. doi: 10.1083/jcb.118.1.63.
5
The folate receptor works in tandem with a probenecid-sensitive carrier in MA104 cells in vitro.在体外，叶酸受体与MA104细胞中对丙磺舒敏感的载体协同发挥作用。
J Clin Invest. 1991 Apr;87(4):1442-9. doi: 10.1172/JCI115150.
6
7
Variant GPI structure in relation to membrane-associated functions of a murine folate receptor.与小鼠叶酸受体膜相关功能有关的糖基磷脂酰肌醇（GPI）结构变体
Biochemistry. 1996 Dec 17;35(50):16305-12. doi: 10.1021/bi961098q.
8
Folate receptors targeted to clathrin-coated pits cannot regulate vitamin uptake.靶向网格蛋白包被小窝的叶酸受体无法调节维生素摄取。
Proc Natl Acad Sci U S A. 1995 Apr 25;92(9):3824-8. doi: 10.1073/pnas.92.9.3824.
9
A review of folate receptor alpha cycling and 5-methyltetrahydrofolate accumulation with an emphasis on cell models in vitro.叶酸受体α循环与5-甲基四氢叶酸积累的综述，重点关注体外细胞模型。
Adv Drug Deliv Rev. 2004 Apr 29;56(8):1085-97. doi: 10.1016/j.addr.2004.01.002.
10
Folate receptor allows cells to grow in low concentrations of 5-methyltetrahydrofolate.叶酸受体使细胞能够在低浓度的5-甲基四氢叶酸中生长。
Proc Natl Acad Sci U S A. 1992 Jul 1;89(13):6006-9. doi: 10.1073/pnas.89.13.6006.
引用本文的文献
1
Transcytosis of LDL Across Arterial Endothelium: Mechanisms and Therapeutic Targets.低密度脂蛋白跨动脉内皮的转胞吞作用：机制与治疗靶点
Arterioscler Thromb Vasc Biol. 2025 Apr;45(4):468-480. doi: 10.1161/ATVBAHA.124.321549. Epub 2025 Feb 27.
2
Exosomes from uterine fluid promote capacitation of human sperm.羊水中的外泌体促进人精子获能。
PeerJ. 2024 Apr 25;12:e16875. doi: 10.7717/peerj.16875. eCollection 2024.
3
Phosphoinositide species and filamentous actin formation mediate engulfment by senescent tumor cells.磷脂酰肌醇种类和丝状肌动蛋白形成介导衰老肿瘤细胞的吞噬作用。
PLoS Biol. 2022 Oct 24;20(10):e3001858. doi: 10.1371/journal.pbio.3001858. eCollection 2022 Oct.
4
The HPV16E7 Affibody as a Novel Potential Therapeutic Agent for Treating Cervical Cancer Is Likely Internalized through Dynamin and Caveolin-1 Dependent Endocytosis.HPV16E7 亲和体作为一种新型潜在的治疗宫颈癌的治疗药物，可能通过网格蛋白依赖内吞作用和动力蛋白依赖性内吞作用被内化。
Biomolecules. 2022 Aug 12;12(8):1114. doi: 10.3390/biom12081114.
5
Clusters of apoptotic signaling molecule-enriched rafts, CASMERs: membrane platforms for protein assembly in Fas/CD95 signaling and targets in cancer therapy.凋亡信号分子富集脂筏簇，CASMERs：Fas/CD95 信号转导中蛋白质组装的膜平台和癌症治疗中的靶标。
Biochem Soc Trans. 2022 Jun 30;50(3):1105-1118. doi: 10.1042/BST20211115.
6
Sterols lower energetic barriers of membrane bending and fission necessary for efficient clathrin-mediated endocytosis.甾醇降低了膜弯曲和裂变的能量障碍，这对于有效的网格蛋白介导的内吞作用是必要的。
Cell Rep. 2021 Nov 16;37(7):110008. doi: 10.1016/j.celrep.2021.110008.
7
Disrupts VE-Cadherin Intercellular Junctions and Traverses Endothelial Barriers Using a Cholesterol-Dependent Mechanism.通过胆固醇依赖性机制破坏VE-钙黏蛋白细胞间连接并穿越内皮屏障。
Front Microbiol. 2021 Jul 20;12:691731. doi: 10.3389/fmicb.2021.691731. eCollection 2021.
8
The Protein Toxins Ricin and Shiga Toxin as Tools to Explore Cellular Mechanisms of Internalization and Intracellular Transport.蛋白毒素蓖麻毒素和志贺毒素作为探索内化和细胞内运输细胞机制的工具。
Toxins (Basel). 2021 May 25;13(6):377. doi: 10.3390/toxins13060377.
9
A matricellular protein fibulin-4 is essential for the activation of lysyl oxidase.基质细胞蛋白纤连蛋白-4对于赖氨酰氧化酶的激活是必不可少的。
Sci Adv. 2020 Nov 25;6(48). doi: 10.1126/sciadv.abc1404. Print 2020 Nov.
10
Targeting Caveolin-1 and Claudin-5 with AY9944, Improve Blood-Brain Barrier Permeability; Computational Simulation and Experimental Study.靶向窖蛋白-1 和闭合蛋白-5 的 AY9944，改善血脑屏障通透性：计算模拟与实验研究。
Cell Mol Neurobiol. 2022 May;42(4):1125-1139. doi: 10.1007/s10571-020-01004-z. Epub 2020 Nov 21.
本文引用的文献
1
Modifications of anionic-lipid domains preceding membrane fusion in guinea pig sperm.豚鼠精子膜融合前阴离子脂质结构域的修饰
J Cell Biol. 1982 Mar;92(3):604-15. doi: 10.1083/jcb.92.3.604.
2
Non-coated membrane invaginations are involved in binding and internalization of cholera and tetanus toxins.无包被膜内陷参与霍乱毒素和破伤风毒素的结合与内化过程。
Nature. 1982 Apr 15;296(5858):651-3. doi: 10.1038/296651a0.
3
Heterogeneous distribution of filipin--cholesterol complexes across the cisternae of the Golgi apparatus.菲律宾菌素-胆固醇复合物在高尔基体潴泡间的异质性分布。
Proc Natl Acad Sci U S A. 1981 Jan;78(1):293-7. doi: 10.1073/pnas.78.1.293.
4
Anionic lipid domains: correlation with functional topography in a mammalian cell membrane.阴离子脂质结构域：与哺乳动物细胞膜功能拓扑结构的相关性
Proc Natl Acad Sci U S A. 1980 Nov;77(11):6601-5. doi: 10.1073/pnas.77.11.6601.
5
Ultrastructural analysis of crystalloid endoplasmic reticulum in UT-1 cells and its disappearance in response to cholesterol.UT-1细胞中晶体样内质网的超微结构分析及其对胆固醇反应时的消失
J Cell Sci. 1983 Sep;63:1-20. doi: 10.1242/jcs.63.1.1.
6
Rings of membrane sterols surround the openings of vesicles and fenestrae, in capillary endothelium.在毛细血管内皮中，膜固醇环围绕着囊泡和窗孔的开口。
J Cell Biol. 1983 Nov;97(5 Pt 1):1592-600. doi: 10.1083/jcb.97.5.1592.
7
Detection of microdomains in biomembranes. An appraisal of recent developments in freeze-fracture cytochemistry.生物膜中微区的检测。对冷冻断裂细胞化学近期进展的评估。
Biochim Biophys Acta. 1983 Aug 11;737(3-4):373-408. doi: 10.1016/0304-4157(83)90007-2.
8
Receptor-mediated endocytosis of low-density lipoprotein in cultured cells.培养细胞中低密度脂蛋白的受体介导内吞作用。
Methods Enzymol. 1983;98:241-60. doi: 10.1016/0076-6879(83)98152-1.
9
10
Isolation and characterization of a folate receptor from human placenta.人胎盘叶酸受体的分离与鉴定
J Biol Chem. 1981 Sep 25;256(18):9684-92.
Zotero 插件